Abstract | PURPOSE: METHODS: IC50 values ( drug concentration inhibiting cell growth by 50%) of the anticancer agents were calculated using XTT cell proliferation assay. Changes in mitochondrial membrane potential ( MMP) were determined using JC-1 assay kit. Changes in the mRNA levels of SK-1 and GCS genes were measured by using RT-PCR technique. RESULTS: CONCLUSION: These results show for the first time that these agents induce apoptosis in MCF-7 cells by downregulating the antiapoptotic SK-1 and GCS genes that may result in accumulation of apoptotic ceramides.
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Authors | G Gucluler, O Piskin, Y Baran |
Journal | Journal of B.U.ON. : official journal of the Balkan Union of Oncology
(J BUON)
2011 Oct-Dec
Vol. 16
Issue 4
Pg. 646-51
ISSN: 1107-0625 [Print] Cyprus |
PMID | 22331716
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Glucosylceramides
- Phosphotransferases (Alcohol Group Acceptor)
- sphingosine kinase
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects, genetics)
- Breast Neoplasms
(drug therapy, genetics, metabolism, pathology)
- Cell Line, Tumor
- Down-Regulation
(drug effects)
- Female
- Glucosylceramides
(genetics)
- Humans
- Membrane Potential, Mitochondrial
(drug effects)
- Phosphotransferases (Alcohol Group Acceptor)
(genetics)
- Real-Time Polymerase Chain Reaction
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