Abstract | BACKGROUND AND AIMS: METHODS: Soluble proteins were extracted from stool samples of 50 participating patients, including those diagnosed with Ulcerative Colitis, Crohn's Disease or IBS, and volunteers with no known intestinal problems. Calprotectin was assessed in the extracted material using the "desk top" Bühlmann Quantum Blue Reader® or by standard ELISA techniques. RESULTS: The mean concentration of calprotectin in the IBD patients group was significantly higher than the mean concentration found in IBS patients and healthy controls (p=0.01). Calprotectin concentrations in IBS patients and controls were indistinguishable. IBD patients that had undergone recent surgery displayed scores similar to controls and IBS patients. Excluding these patients yielded a specificity of 100% for results from both CD and UC patients and an accuracy rate of 1 for CD and 0.89 for UC patients in ROC analysis. Quantum Blue Reader® calprotectin levels were available within 30 min and correlated well with results derived from standard ELISA assays, which took over 8h to complete. CONCLUSION: Our results confirm the effective use of fecal calprotectin levels in differentiating non-inflammatory from active inflammatory intestinal diseases. The desk top Bühlmann Quantum Blue Reader® exhibits a fast, non-invasive, and reliable way of identifying an inflammatory intestinal disease.
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Authors | Michael J Sydora, Beate C Sydora, Richard N Fedorak |
Journal | Journal of Crohn's & colitis
(J Crohns Colitis)
Vol. 6
Issue 2
Pg. 207-14
(Mar 2012)
ISSN: 1876-4479 [Electronic] England |
PMID | 22325175
(Publication Type: Comparative Study, Journal Article, Validation Study)
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Copyright | Copyright © 2011 European Crohn's and Colitis Organisation. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Biomarkers
- Leukocyte L1 Antigen Complex
|
Topics |
- Adult
- Aged
- Aged, 80 and over
- Biomarkers
(analysis)
- Colitis, Ulcerative
(diagnosis)
- Crohn Disease
(diagnosis)
- Diagnosis, Differential
- Enzyme-Linked Immunosorbent Assay
- Feces
(chemistry)
- Female
- Humans
- Irritable Bowel Syndrome
(diagnosis)
- Leukocyte L1 Antigen Complex
(analysis)
- Male
- Middle Aged
- Point-of-Care Systems
- Sensitivity and Specificity
- Statistics, Nonparametric
- Young Adult
|