Activated
carbon characteristically shows an extremely high transmigration to the lymph node, as well as a sustained release of the adsorbed drugs. Therefore, several attempts to use activated
carbon as a carrier in
cancer chemotherapy have been done. In the present study, we first introduced a new
drug-
dosage form of
cisplatin (CDDP) adsorbed to activated
carbon particles (CDDP-CH), and examined its characteristics and anti-
cancer effect against human
bladder cancer cell lines. CDDP
solution of varied concentrations (Randa) was mixed with activated
carbon particles (
Norit A) and examined for adsorption and discharge by atomic absorption spectrophotometry and high performance liquid chromatography. Total CDDP adsorption increased in proportion to the amount of activated
carbon. CDDP-CH was successfully prepared at an efficient concentration for
cancer chemotherapy and CDDP-CH slowly discharged CDDP, indicating it as a useful means for the anti-
cancer drug. Using cultured human
bladder cancer cell lines (KU-1, HTB9), the anti-
cancer effect was compared between CDDP and CDDP-CH by MTT-assay and double layer soft
agar colony assay. CDDP-CH revealed an inhibitory effect against human
bladder cancer cell lines. In view of the fact that activated
carbon readily migrates to the lymph node, clinical application of this
drug dosage form may be useful in cases of malignant
tumor metastasis to the lymph node.