Pre-existent Hsp72 contributes to glutamine-induced hepatic hsp72 gene activation during heat shock recovery period in rat.

Abstract

SCOPE:

Functional maintenance of liver is very important at all times for personal health. Hsp induction is associated with the protection of the organ. Glutamine, a nutrient inducer of Hsps, enhances cellular survival via Hsp72 induction in several organs, but not in the liver. The present study showed a novel approach to facilitate glutamine-induced hepatic Hsp72 synthesis and its possible mechanisms were discussed.

METHODS AND RESULTS:

Sprague-Dawley rats were used as the experimental animals and the livers were the targets. Glutamine was administered via tail-vein injection, and its effects on hsp72 gene activation, including Hsp72 expression, heat shock factor-1 (HSF-1) phosphorylation and DNA-binding activation, were evaluated. The results showed that Hsp72 itself played a critical role in glutamine-induced hepatic Hsp72 synthesis during HS recovery period in a dose-dependent manner of preexistent Hsp72. The peak value of HSF-1 phosphorylation, HSF-1 DNA-binding activity, hsp72 mRNA accumulation, and Hsp72 synthesis was detected at 8 h after glutamine administration.

CONCLUSION:

Glutamine switched on alteration pathway in inducing hsp72 gene activation. The existence of Hsp72 plays a critical role in the reactivation of hsp72 gene. Glutamine sustained the induction of intracellular Hsp72, which could be beneficial in protecting the liver from various injuries.

• Authors: Shu-Jung Wang, Hsiang-Wen Chen, Rei-Cheng Yang
• Journal: Molecular nutrition & food research (Mol Nutr Food Res) Vol. 56 Issue 3 Pg. 410-6 (Mar 2012) ISSN: 1613-4133 [Electronic] Germany
• PMID: 22319056 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• DNA-Binding Proteins
• HSP72 Heat-Shock Proteins
• Heat Shock Transcription Factors
• RNA, Messenger
• Transcription Factors
• Glutamine

Topics

• Animals
• Blotting, Western
• DNA-Binding Proteins (genetics, metabolism)
• Gene Expression Regulation
• Glutamine (pharmacology)
• HSP72 Heat-Shock Proteins (genetics, metabolism)
• Heat Shock Transcription Factors
• Heat-Shock Response (drug effects)
• Liver (metabolism)
• Male
• Phosphorylation
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction (methods)
• Transcription Factors (genetics, metabolism)