Modulation of activated leukocyte cell adhesion molecule-mediated invasion triggers an innate immune gene response in melanoma.

Abstract

Activated leukocyte cell adhesion molecule (ALCAM/CD166) is a progression marker of a variety of cancers, including melanoma, and is a marker for mesenchymal stem cells. ALCAM expression triggers matrix metalloproteinase activity and correlates with the transition between superficial melanoma growth and deep dermal invasion in vivo. We previously showed that manipulating ALCAM functionality could both decrease and increase melanoma invasion, depending on the manner by which ALCAM function was altered. How ALCAM exerts these opposing invasive phenotypes remained elusive. In the present study, we analyzed differences in melanoma cell gene expression in two- and three-dimensional cultures as function of ALCAM-mediated adhesion. We identified a cluster of genes highly responsive to ALCAM functionality and relevant for melanoma invasion. This cluster is characterized by known invasion-related genes similar to L1 neuronal cell adhesion molecule and showed a remarkable induction of several innate immune genes. Unexpectedly, we identified major variations in the expression of genes related to an immunological response when modulating ALCAM function, including complement factors C1r and C1s. The expression and function of these proteinases were confirmed in protein assays and in vivo. Together, our results demonstrate a link between ALCAM functionality and the immune transcriptome, and support the assumption that ALCAM-ALCAM interactions could function as a cell signaling complex to promote melanoma tumor invasion.

• Authors: Jeroen W J van Kilsdonk, Nozomi Takahashi, Ulrich Weidle, Helmut Burtscher, Jonathan Jarry, Mohamed R Daha, Guido W M Swart, Léon C L T van Kempen
• Journal: The Journal of investigative dermatology (J Invest Dermatol) Vol. 132 Issue 5 Pg. 1462-70 (May 2012) ISSN: 1523-1747 [Electronic] United States
• PMID: 22318386 (Publication Type: Journal Article)

Chemical References

• Activated-Leukocyte Cell Adhesion Molecule
• RNA, Messenger
• Complement C1r
• Complement C1s

Topics

• Activated-Leukocyte Cell Adhesion Molecule (genetics, metabolism)
• Cell Adhesion
• Cell Count
• Complement C1r (metabolism)
• Complement C1s (metabolism)
• Gene Expression Profiling
• Humans
• Immunity, Innate (genetics)
• Melanoma (genetics, immunology, metabolism)
• Microarray Analysis
• Neoplasm Invasiveness (genetics)
• Neoplasm Metastasis (genetics)
• Phenotype
• RNA, Messenger (metabolism)
• Skin Neoplasms (genetics, immunology, metabolism)
• Tumor Cells, Cultured
• Up-Regulation