HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Lack of association between aspirin-triggered 15-hydroxyeicosatetraenoic acid release and mast cell/eosinophil activation in nasal polyps from aspirin-sensitive patients.

AbstractBACKGROUND:
The mechanism of aspirin sensitivity in patients with asthma and rhinosinusitis has been attributed to arachidonic acid metabolism abnormalities.
OBJECTIVE:
We aimed to test whether aspirin-triggered generation of 15-hydroxyeicosatetraenoic acid (15-HETE) in nasal polyp dispersed cells (NPDCs) from aspirin-sensitive patients is associated with activation of inflammatory cells.
METHODS:
Polyps were obtained from 11 aspirin-sensitive and 19 aspirin-tolerant patients with chronic rhinosinusitis. NPDCs were stimulated by aspirin or calcium ionophore. Levels of 15-HETE, leukotriene (LT) C4, eosinophil cationic protein (ECP), and tryptase were measured in NPDC supernatant.
RESULTS:
NPDCs from aspirin-sensitive patients contained more eosinophils (14% vs 9%, P < .05) and released 2.4-fold more ECP (P < .01) at baseline. Stimulation with aspirin (200 microM) resulted in a significant increase in 15-HETE generation only in tissue from aspirin-sensitive patients (mean increase, 82%) but did not induce any increase in the release of LTC4, ECP, or tryptase. Preincubation with calcium ionophore resulted in significantly enhanced generation of 15-HETE, ECP, tryptase, and LTC4 in patients from both groups. Incubation of NPDCs with misoprostol inhibited aspirin-induced 15-HETE generation in aspirin-sensitive patients and calcium ionophore-induced 15-HETE, ECP, and tryptase release in both aspirin-sensitive and aspirin-tolerant patients.
CONCLUSION:
Our study demonstrated that aspirin-induced 15-HETE generation in nasal polyps from aspirin-sensitive patients is not associated with activation of mast cells and eosinophils. Misoprostol has a potent inhibitory effect on the activation of cells derived from the site of nasal mucosal inflammation, regardless of sensitivity to aspirin.
AuthorsA Lewandowska-Polak, M Jedrzejczak-Czechowicz, J S Makowska, M Jarzebska, A Jankowski, M L Kowalski
JournalJournal of investigational allergology & clinical immunology (J Investig Allergol Clin Immunol) Vol. 21 Issue 7 Pg. 507-13 ( 2011) ISSN: 1018-9068 [Print] Spain
PMID22312933 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Calcium Ionophores
  • Hydroxyeicosatetraenoic Acids
  • Misoprostol
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid
  • Arachidonate 15-Lipoxygenase
  • Aspirin
Topics
  • Adult
  • Aged
  • Aged, 80 and over
  • Arachidonate 15-Lipoxygenase (physiology)
  • Aspirin (adverse effects)
  • Calcium Ionophores (pharmacology)
  • Drug Hypersensitivity (metabolism)
  • Eosinophils (drug effects, physiology)
  • Female
  • Humans
  • Hydroxyeicosatetraenoic Acids (secretion)
  • Male
  • Mast Cells (drug effects, physiology)
  • Middle Aged
  • Misoprostol (pharmacology)
  • Nasal Polyps (immunology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: