Abstract | OBJECTIVE: To identify the underlying mechanisms of the protective effects of Dingxin Recipe (: , DXR), a Chinese compound prescription that has been used clinically in China for more than 20 years, on ischemia/reperfusion (I/R)-induced arrhythmias in rat model. METHODS: A total of 30 rats were randomly divided into three groups: sham group, I/R group, and DXR-pretreated I/R (DXR-I/R) group. Rats in the DXR-DXRI/R group were intragastrically administrated with DXR (12.5 g/kg per day) for consecutive 7 days, while rats I/in the sham and I/R groups were administrated with normal saline. Arrhythmias were introduced by I/R and electrocardiograms (ECG) were recorded. Two-dimensional (2-D) polyacrylamide gel electrophoresis and matrix-matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to identify assisted differentially expressed proteins. Immunohistochemistry, real-time quantitative polymerase chain reaction (RQ-RQPCR), Western blot, and enzyme-linked immunosorbent assay (ELISA) were performed to analyze proteins PCR), obtained in the above experiments. RESULTS: CONCLUSIONS: DXR could alleviate I/R-induced arrhythmias, which might be related to increased expression of PHB. The enhanced expression of PHB prevented against the depletion of GSH and consequently inhibited apoptosis of cardiomyocytes. Furthermore, up-regulation of PHB might ameliorate I/R-induced cell death and leakage of hFABP by suppressing neutrophil infiltration and IL-6 expressions.
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Authors | Yu-Hua Jia, Yun-Xian Zhang, Li-Jun Li, Ya-Wei Liu, Chun-Hua Li, Xiu-Qiong Fu, Ping Zeng, Wei-Kang Wu, Xue-Gang Sun |
Journal | Chinese journal of integrative medicine
(Chin J Integr Med)
Vol. 18
Issue 2
Pg. 120-9
(Feb 2012)
ISSN: 1672-0415 [Print] China |
PMID | 22311408
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Drugs, Chinese Herbal
- FABP3 protein, rat
- Fatty Acid Binding Protein 3
- Fatty Acid-Binding Proteins
- Interleukin-6
- Phb protein, rat
- Prohibitins
- Repressor Proteins
- dingxin
- Glutathione
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Topics |
- Animals
- Arrhythmias, Cardiac
(etiology, prevention & control)
- Drugs, Chinese Herbal
(pharmacology, therapeutic use)
- Electrophoresis, Gel, Two-Dimensional
- Fatty Acid Binding Protein 3
- Fatty Acid-Binding Proteins
(metabolism)
- Glutathione
(metabolism)
- Heart Ventricles
(drug effects, metabolism, pathology)
- Immunohistochemistry
- Inflammation
(complications, metabolism, pathology)
- Interleukin-6
(metabolism)
- Male
- Myocardial Infarction
(complications, drug therapy, pathology)
- Neutrophil Infiltration
(drug effects)
- Peptide Mapping
- Prohibitins
- Proteomics
- Rats
- Rats, Wistar
- Reperfusion Injury
(complications)
- Repressor Proteins
(metabolism)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Spectrophotometry
- Up-Regulation
(drug effects)
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