Metabolism of hyperpolarized [1-¹³C]pyruvate in the isolated perfused rat lung - an ischemia study.

Abstract

We report studies of the effect of ischemia on the metabolic activity of the intact perfused lung and its restoration after a period of reperfusion. Two groups of rat lungs were studied using hyperpolarized 1-(13) C pyruvate to compare the rate of lactate labeling differing only in the temporal ordering of ischemic and normoxic acquisitions. In both cases, a several-fold increase in lactate labeling was observed immediately after a 25-min ischemia event as was its reversal back to the baseline after 30-40 min of resumed perfusion (n = 5, p < 0.025 for both comparisons). These results were corroborated by (31) P spectroscopy and correspond well to measured changes in lactate pool size determined by (1) H spectroscopy of freeze-clamped specimens.

• Authors: B Pullinger, H Profka, J H Ardenkjaer-Larsen, N N Kuzma, S Kadlecek, R R Rizi
• Journal: NMR in biomedicine (NMR Biomed) Vol. 25 Issue 10 Pg. 1113-8 (Oct 2012) ISSN: 1099-1492 [Electronic] England
• PMID: 22311307 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2012 John Wiley & Sons, Ltd.

Chemical References

• Carbon Isotopes
• Lactic Acid
• Pyruvic Acid

Topics

• Animals
• Carbon Isotopes
• In Vitro Techniques
• Ischemia (metabolism)
• Isotope Labeling
• Lactic Acid (metabolism)
• Lung (metabolism)
• Magnetic Resonance Spectroscopy
• Male
• Perfusion (methods)
• Principal Component Analysis
• Pyruvic Acid (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reperfusion