MG132 as a
proteasome inhibitor can induce apoptotic cell death in
lung cancer cells. However, little is known about the toxicological cellular effects of
MG132 on normal primary lung cells. Here, we investigated the effects of N-acetyl
cysteine (NAC) and
vitamin C (well known
antioxidants) or L-
buthionine sulfoximine (BSO; an inhibitor of GSH synthesis) on MG132-treated human pulmonary fibroblast (HPF) cells in relation to cell death,
reactive oxygen species (ROS) and
glutathione (GSH).
MG132 induced growth inhibition and death in HPF cells, accompanied by the loss of mitochondrial membrane potential (
MMP; ∆ψm).
MG132 increased ROS levels and GSH-depleted cell numbers in HPF cells. Both
antioxidants, NAC and
vitamin C, prevented growth inhibition, death and
MMP (∆ψm) loss in MG132-treated HPF cells and also attenuated ROS levels in these cells. BSO showed a strong increase in ROS levels in MG132-treated HPF cells and slightly enhanced the growth inhibition, cell death,
MMP (∆ψm) loss and GSH depletion. In addition, NAC decreased anonymous ubiquitinated
protein levels in MG132-treated HPF cells. Furthermore,
superoxide dismutase (SOD) 2,
catalase (CTX) and GSH
peroxidase (GPX) siRNAs enhanced HPF cell death by
MG132, which was not correlated with ROS and GSH level changes. In conclusion,
MG132 induced the growth inhibition and death of HPF cells, which were accompanied by increasing ROS levels and GSH depletion. Both NAC and
vitamin C attenuated HPF cell death by
MG132, whereas BSO slightly enhanced the death.