Dopamine release via the vacuolar ATPase V0 sector c-subunit, confirmed in N18 neuroblastoma cells, results in behavioral recovery in hemiparkinsonian mice.

Abstract

A 16-kDa proteolipid, mediatophore, in Torpedo electric organs mediates Ca(2+)-dependent acetylcholine release. Mediatophore is identical to the pore-forming stalk c-subunit of the V0 sector of vacuolar proton ATPase (ATP6V0C). The function of ATP6V0C in the mammalian central nervous system is not clear. Here, we report transfection of adeno-associated viral vectors harboring rat ATP6V0C into the mouse substantia nigra, in which high potassium stimulation increased overflow of endogenous dopamine (DA) measured in the striatum by in vivo microdialysis. Next, in the striatum of 6-hydroxydopamine-lesioned mice, a model of Parkinson's disease (PD), human tyrosine hydroxylase, aromatic l-amino-acid decarboxylase and guanosine triphosphate cyclohydrolase 1, together with or without ATP6V0C, were expressed in the caudoputamen for rescue. Motor performance on the accelerating rotarod test and amphetamine-induced ipsilateral rotation were improved in the rescued mice coexpressing ATP6V0C. [(3)H]DA, taken up into cultured N18 neuronal tumor cells transformed to express ATP6V0C, was released by potassium stimulation. These results indicated that ATP6V0C mediates DA release from nerve terminals in the striatum of DA neurons of normal mice and from gene-transferred striatal cells of parkinsonian mice. The results suggested that ATP6V0C may be useful as a rescue molecule in addition to DA-synthetic enzymes in the gene therapy of PD.

• Authors: Duo Jin, Shin-Ichi Muramatsu, Nobuaki Shimizu, Shigeru Yokoyama, Hirokazu Hirai, Kiyofumi Yamada, Hong-Xiang Liu, Chiharu Higashida, Minako Hashii, Akihiko Higashida, Masahide Asano, Shoji Ohkuma, Haruhiro Higashida
• Journal: Neurochemistry international (Neurochem Int) Vol. 61 Issue 6 Pg. 907-12 (Nov 2012) ISSN: 1872-9754 [Electronic] England
• PMID: 22265874 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ltd. All rights reserved.

Chemical References

• Vacuolar Proton-Translocating ATPases
• Dopamine

Topics

• Animals
• Behavior, Animal
• Cell Line, Tumor
• Dopamine (metabolism)
• Male
• Mice
• Mice, Inbred ICR
• Microdialysis
• Neuroblastoma (enzymology, metabolism, pathology)
• Parkinson Disease (physiopathology)
• Vacuolar Proton-Translocating ATPases (metabolism)