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Expression of N-methyl-d-aspartate receptor 1 and its phosphorylated state in basal ganglia of a neonatal piglet hypoxic-ischemic brain injury model: a controlled study of (1)H MRS.

Abstract
Excitatory amino acids (EAAs) and excitotoxicity medicated by receptors of these amino acids play an important role in hypoxic-ischemic brain injury (HIBI), but most studies were ex vivo experiments, the mechanism in vivo is not well understood. We sought to study the expression of N-methyl-d-aspartate receptor 1 (NR1) and phosphorylated N-methyl-d-aspartate receptor 1 (P-NR1) in basal ganglia in a piglet model of HIBI and to investigate the correlation between Glx(Glu/Gln) value measured by magnetic resonance spectroscopy (MRS) and NR1/P-NR1 expression. Multi-voxel (1)H MRS was applied to detect change in Glx in basal ganglia of the newborn piglets in vivo. Automatic amino acid analyzer was applied to accurately quantify the Glu concentration. Immunohistochemical method was used to examine the expression of NR1 and P-NR1. The NR1 receptors in basal ganglia of the newborn piglets were significantly activated after HIBI. P-NR1 expression in the basal ganglia was consistent with the change in brain Glu content, so the activation status of NMDA receptor in the brain could be indirectly reflected by β-, γ-Glx/NAA measured by (1)H MRS.
AuthorsXiao-Yu Wang, Hong-Wei Wang, Xi-Hu Fu, Wei-Qing Zhang, Xiu-Ying Wu, Qi-Yong Guo, Xiao-Ming Wang
JournalEuropean journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society (Eur J Paediatr Neurol) Vol. 16 Issue 5 Pg. 492-500 (Sep 2012) ISSN: 1532-2130 [Electronic] England
PMID22261079 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2012 European Paediatric Neurology Society. Published by Elsevier Ltd. All rights reserved.
Chemical References
  • NR1 NMDA receptor
  • Receptors, N-Methyl-D-Aspartate
  • Glutamine
  • Glutamic Acid
Topics
  • Animals
  • Animals, Newborn
  • Basal Ganglia (metabolism, pathology)
  • Glutamic Acid (metabolism)
  • Glutamine (metabolism)
  • Hypoxia-Ischemia, Brain (metabolism, pathology)
  • Magnetic Resonance Spectroscopy
  • Neurons (metabolism, pathology)
  • Phosphorylation
  • Receptors, N-Methyl-D-Aspartate (metabolism)
  • Swine

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