Abstract | BACKGROUND: OBJECTIVE: To elucidate the effect of that point mutation on skin barrier structures and functions, we generated mice with a point mutation of R142C, which corresponds to the R143C mutation in human TG1. METHODS: A mouse line with the R142C point mutation in TG1 was established using a gene targeting technique and the Cre-loxP system. The skin phenotypes were analyzed in homozygous mutant Tgm1(R142C/R142C) mice. RESULTS: In the skin of Tgm1(R142C/R142C) mice, expression of the mutant transcripts was comparable with wild-type or Tgm1(+/R142C) mice. However, the amount of mutated protein in the skin was markedly decreased in Tgm1(R142C/R142C) mice, and the TG1 activity of Tgm1(R142C/R142C) keratinocytes was almost lost. Tgm1(R142C/R142C) mice exhibited morphological and functional skin barrier defects and neonatal lethality. The stratum corneum of those mice lacked cornified envelopes, and loricrin, the major structural component, failed to assemble at the corneocyte cell periphery. Tgm1(R142C/R142C) mice showed a marked increase in transepidermal water loss and their skin was easily permeable to toluidine blue dye. The intercellular lipid lamellar structures of the stratum corneum were irregular and the 13-nm periodic X-ray diffractions from the stratum corneum lipid molecules were lost in vivo. CONCLUSION: From these results, we suggest that the R142C mutation of TG1 reduces the enzyme stability which is indispensable for development of the stratum corneum and skin barrier function and for postnatal survival of mice.
|
Authors | Noboru Nakagawa, Masaaki Yamamoto, Yasutomo Imai, Yoshiko Sakaguchi, Takami Takizawa, Noboru Ohta, Naoto Yagi, Ichiro Hatta, Kiyotaka Hitomi, Toshihiro Takizawa, Junji Takeda, Tatsuya Tsuda, Masato Matsuki, Kiyofumi Yamanishi |
Journal | Journal of dermatological science
(J Dermatol Sci)
Vol. 65
Issue 3
Pg. 196-206
(Mar 2012)
ISSN: 1873-569X [Electronic] Netherlands |
PMID | 22258055
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright © 2011 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
- Transglutaminases
- transglutaminase 1
|
Topics |
- Animals
- Animals, Newborn
(physiology)
- Disease Models, Animal
- Epidermis
(embryology, enzymology, pathology)
- Gene Knock-In Techniques
- Ichthyosis
(genetics, metabolism)
- Mice
- Mice, Transgenic
- Phenotype
- Point Mutation
(genetics)
- Survival Rate
- Transglutaminases
(genetics, metabolism)
- X-Ray Diffraction
|