In vitro targeting of avidin-expressing glioma cells with biotinylated persistent luminescence nanoparticles.

Abstract

Far red emitting persistent luminescence nanoparticles (PLNP) were synthesized and functionalized with biotin to study their targeting ability toward biotin-binding proteins. First, the interaction of biotin-decorated PLNP with streptavidin, immobilized on a plate, was shown to be highly dependent on the presence of a PEG spacer between the surface of the nanoparticles and the biotin ligand. Second, interaction between biotin-PEG-PLNP and free neutravidin in solution was confirmed by fluorescence microscopy. Finally, in vitro binding study on BT4C cells expressing lodavin fusion protein, bearing the extracellular avidin moiety, showed that such biotin-covered PLNP could successfully be targeted to malignant glioma cells through a specific biotin-avidin interaction. The influence of nanoparticle core diameter, incubation time, and PLNP concentration on the efficiency of targeting is discussed.

• Authors: Thomas Maldiney, Minna U Kaikkonen, Johanne Seguin, Quentin le Masne de Chermont, Michel Bessodes, Kari J Airenne, Seppo Ylä-Herttuala, Daniel Scherman, Cyrille Richard
• Journal: Bioconjugate chemistry (Bioconjug Chem) Vol. 23 Issue 3 Pg. 472-8 (Mar 21 2012) ISSN: 1520-4812 [Electronic] United States
• PMID: 22250884 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Avidin
• Biotin

Topics

• Animals
• Avidin (metabolism)
• Biotin (metabolism)
• Brain Neoplasms (metabolism, pathology)
• Cell Line, Tumor
• Fluorescent Antibody Technique
• Glioma (metabolism, pathology)
• In Vitro Techniques
• Luminescence
• Microscopy, Fluorescence
• Nanoparticles
• Rats