[Expression of RUNX3 in cervical carcinoma and its clinical significance].

Abstract

OBJECTIVE To explore the role of runt-related transcription factor 3(RUNX3) in the tumorgenesis and progression of cervical carcinoma. METHODS The immunohistochemical staining technique was used to detect the expression of RUNX3 protein in 25 cases of normal cervix, 34 intraepithelia neoplasia (CIN), and 48 cervical carcinomas. SYBR Green I chimeric fluorescence Real-time PCR was applied to detect the expression of RUNX3 mRNA in 10 cases of normal cervix, 24 CIN, and 30 cervical carcinomas. RESULTS The expressions of RUNX3 protein and mRNA in normal cervix, CINI,CINII-III, and cervical carcinoma tissues tended to be down-regulated. There was significant difference among these groups (P<0.05). The expressions of RUNX3 protein and mRNA in the cervical carcinoma tissues were correlated with the histological differentiation, clinical stage, and lymphatic metastasis (P<0.05), but had no relationship with the age, high-risk human papillomavirus infection, and histological classification (P> 0.05). CONCLUSION RUNX3 may function as a tumor suppressor gene in the occurrence and progression of cervical carcinoma.

• Authors: Yuping Deng, Meifang Nie, Fengying Liu, Shan Jiang, Yizhi Liu, Dengqing Li, Qucheng Meng, Jin Li, Minzhu Huang, Mingming Wang
• Journal: Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences (Zhong Nan Da Xue Xue Bao Yi Xue Ban) Vol. 36 Issue 12 Pg. 1189-94 (Dec 2011) ISSN: 1672-7347 [Print] China
• PMID: 22246363 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Core Binding Factor Alpha 3 Subunit
• RNA, Messenger
• Runx3 protein, human

Topics

• Adult
• Carcinoma, Squamous Cell (genetics, metabolism)
• Core Binding Factor Alpha 3 Subunit (genetics, metabolism)
• Disease Progression
• Female
• Humans
• Middle Aged
• RNA, Messenger (genetics, metabolism)
• Uterine Cervical Neoplasms (genetics, metabolism)
• Uterine Cervical Dysplasia (genetics, metabolism)