BML-111, a lipoxin receptor agonist, protects haemorrhagic shock-induced acute lung injury in rats.

Abstract

OBJECTIVES:

The main pathogenesis of acute lung injury induced by haemorrhagic shock is inflammation. BML-111, a lipoxinA(4)-receptor agonist, promotes acute inflammatory resolution. We sought to elucidate whether BML-111 protects haemorrhagic shock-induced acute lung injury in rats.

METHODS:

Thirty two adult male rats were randomized to sham group (sham), haemorrhagic shock/resuscitation (HS), HS plus BML-111 (BML-111), and HS plus BML-111 and BOC-2 (BOC-2). Haemorrhagic shock was induced by blood drawing, and then resuscitation was obtained by infusion of shed blood and two-fold volume saline.

RESULTS:

Histological findings, as well as assays of neutrophilic infiltration (myeloperoxidase activity, ICAM-1 expression), inflammatory cytokines and pro-inflammatory factor (IκB-α and NF-κB p65) confirmed that haemorrhagic shock induced acute lung injury. BML-111 significantly mitigated acute lung injury induced by haemorrhagic shock. However, BOC-2, an antagonist of the lipoxinA(4)-receptor, partially reversed the protective effect of BML-111 on the haemorrhagic shock-induced the acute lung injury.

CONCLUSION:

BML-111 protects haemorrhagic shock-induced acute lung injury in rats.

• Authors: Jie Gong, Si Guo, Hong-Bin Li, Shi-Ying Yuan, You Shang, Shang-Long Yao
• Journal: Resuscitation (Resuscitation) Vol. 83 Issue 7 Pg. 907-12 (Jul 2012) ISSN: 1873-1570 [Electronic] Ireland
• PMID: 22245750 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• 5(S),6(R)-7-trihydroxyheptanoic acid, methyl ester
• Cell Adhesion Molecules
• Heptanoic Acids
• NF-kappa B
• Oligopeptides
• Receptors, Lipoxin
• butyloxycarbonyl-phenylalanyl-leucyl-phenylalanyl-leucyl-phenylalanine

Topics

• Acute Lung Injury (prevention & control)
• Animals
• Blotting, Western
• Cell Adhesion Molecules (metabolism)
• Electrophoretic Mobility Shift Assay
• Heptanoic Acids (pharmacology, therapeutic use)
• Lung (drug effects, metabolism, pathology)
• Male
• NF-kappa B (metabolism)
• Oligopeptides (pharmacology)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Receptors, Lipoxin
• Resuscitation
• Shock, Hemorrhagic (complications, drug therapy, pathology)