Characterization of a tumor cell surface protein with heterologous antisera to a spontaneous BALB/c lung carcinoma.

Line 1, a spontaneous alveolar carcinoma from a BALB/c mouse, is highly metastatic and weakly antigenic in the syngeneic host. Sera and enriched antibody preparations were made specific for line 1 cells by in vitro and in vivo absorptions. By lactoperoxidase-catalyzed radioiodination of cell surface protein followed by precipitation with specific antibodies, a protein with a molecular weight of about 180,000 (designated TSP-180) was identified that was present on line 1 cells but not on normal lung cells or Moloney sarcoma tumor cells. Studies of competition for immune precipitation of TSP-180 by unlabeled protein preparations from various sources indicate that (a) TSP-180 is present in tumor cells of various culture passage levels, (b) tumor cells grown i.m. also contain TSP-180, and (c) normal lung proteins compete weakly, and only at very high protein concentrations. A protein similar to TSP-180 was detected on Madison 109 carcinoma and on three other lung carcinomas adapted to culture. Experiments with specific antisera show that TSP-180 is not lactoperoxidase, fetal bovine serum protein, large external transformation-sensitive protein, or an antigen related to murine leukemia virus proteins.
AuthorsS J Kennel
JournalCancer research (Cancer Res) Vol. 39 Issue 8 Pg. 2934-9 (Aug 1979) ISSN: 0008-5472 [Print] UNITED STATES
PMID222438 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • Antibodies, Neoplasm
  • Antigens, Neoplasm
  • Antigens, Surface
  • Neoplasm Proteins
  • Adenocarcinoma, Bronchiolo-Alveolar (immunology)
  • Animals
  • Antibodies, Neoplasm
  • Antigens, Neoplasm (isolation & purification)
  • Antigens, Surface (isolation & purification)
  • Cell Line
  • Immunoenzyme Techniques
  • Lung Neoplasms (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Neoplasm Proteins (immunology)
  • Neoplasms, Experimental (immunology)

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