[Study on effect of berberine on modulating lipid and CPT I A gene expression].

Abstract

OBJECTIVE:

To investigate the modulating effect on lipid and gene expressions of CPT I A caused by berberine (Ber) in experimental hyperlipidemia rats.

METHOD:

Male SD rats were randomly divided into 5 groups according to the blood lipid values: normal group, hyperlipidemia group, 300 mg x kg(-1) x d(-1) Ber-treated group, 60 mg x kg(-1) x d(-1) Ber-treated group, and 7.2 mg x kg(-1) x d(-1) lovastatin-treated group. Normal group were fed with base diet and other groups were fed with high fat and cholesterol diet. 12 weeks after drugs were given the TC, TG, LDL-C, and HDL-C from rat blood samples were tested by automatic biochemistry analyzer. Gene expressions of CPT I A and PPARalpha were evaluated by RT-PCR and Western blot, respectively.

RESULT:

It was shown that Ber significantly decreased TC and LDL-C, but increased HDL-C in dose-dependent manner, elevated expressions of CPT I A mRNA and protein without influence on PPARalpha expression. Similar effects from lovastatin on lipidemia were observed except the Ber effect on CPT I A gene expression.

CONCLUSION:

Ber has modulating effect on the lipid metabolism, the mechanism of which may be by promoting the CPT I A gene expression.

• Authors: Hong Wang, Lingyun Shi, Huafeng Yin, Qixin Zhou
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 36 Issue 19 Pg. 2715-8 (Oct 2011) ISSN: 1001-5302 [Print] China
• PMID: 22242436 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drugs, Chinese Herbal
• PPAR gamma
• Berberine
• Carnitine O-Palmitoyltransferase

Topics

• Animals
• Berberine (administration & dosage)
• Carnitine O-Palmitoyltransferase (genetics, metabolism)
• Disease Models, Animal
• Drugs, Chinese Herbal (administration & dosage)
• Gene Expression (drug effects)
• Humans
• Hyperlipidemias (drug therapy, enzymology, genetics, metabolism)
• Lipid Metabolism (drug effects)
• Male
• PPAR gamma (genetics, metabolism)
• Random Allocation
• Rats
• Rats, Wistar