Overexpression of extracellular superoxide dismutase has a protective role against hyperoxia-induced brain injury in neonatal mice.

Abstract

There is increasing evidence that hyperoxia, particularly at the time of birth, may result in neurological injury, in particular to the susceptible vasculature of these tissues. This study was aimed at determining whether overexpression of extracellular superoxide dismutase (EC-SOD) is protective against brain injury induced by hyperoxia. Transgenic (TG) mice (with an extra copy of the human extracellular superoxide dismutase gene) and wild-type (WT) neonate mice were exposed to hyperoxia (95% of F(i) o(2) ) for 7 days after birth versus the control group in room air. Brain positron emission tomography (PET) scanning with fludeoxyglucose (FDG) isotope uptake was performed after exposure. To assess apoptosis induced by hyperoxia exposure, caspase 3 ELISA and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were performed. Quantitative western blot for the following inflammatory markers was performed: glial fibrillary acidic protein, ionized calcium-binding adaptor molecule 1, macrophage-inhibiting factor, and phospho-AMP-activated protein kinase. PET scanning with FDG isotope uptake showed significantly higher uptake in the WT hyperoxia neonate brain group (0.14 ± 0.03) than in both the TG group (0.09 ± 0.01) and the control group (0.08 ± 0.02) (P< 0.05). Histopathological investigation showed more apoptosis and dead neurons in hippocampus and cerebellum brain sections of WT neonate mice after exposure to hyperoxia than in TG mice; this finding was also confirmed by TUNEL staining. The caspase 3 assay confirmed the finding of more apoptosis in WT hyperoxia neonates (0.814 ± 0.112) than in the TG hyperoxic group (0.579 ± 0.144) (P < 0.05); this finding was also confirmed by TUNEL staining. Quantitative western blotting for the inflammatory and metabolic markers showed significantly higher expression in the WT group than in the TG and control groups. Thus, overexpression of EC-SOD in the neonate brain offers significant protection against hyperoxia-induced brain damage.

• Authors: Nahla Zaghloul, Mansoor Nasim, Hardik Patel, Champa Codipilly, Philippe Marambaud, Stephen Dewey, Wynne K Schiffer, Mohamed Ahmed
• Journal: The FEBS journal (FEBS J) Vol. 279 Issue 5 Pg. 871-81 (Mar 2012) ISSN: 1742-4658 [Electronic] England
• PMID: 22240000 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2012 The Authors Journal compilation © 2012 FEBS.

Chemical References

• Biomarkers
• SOD3 protein, human
• Superoxide Dismutase

Topics

• Animals
• Animals, Newborn
• Apoptosis
• Biomarkers (metabolism)
• Blotting, Western
• Brain Injuries (enzymology, etiology, prevention & control)
• Enzyme-Linked Immunosorbent Assay
• Humans
• Hyperoxia (complications, pathology)
• In Situ Nick-End Labeling
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Oxidative Stress
• Positron-Emission Tomography
• Superoxide Dismutase (genetics, metabolism)