Metabolic correction of fucosidosis lymphoid cells by galaptin-alpha-L-fucosidase conjugates.

Abstract

To determine if isolated galaptin, an endogenous galactoside-binding lectin, could serve as a transport vehicle of therapeutic agents to cells, galaptin and alpha-L-fucosidase were coupled using glutaraldehyde. The conjugates were incubated with alpha-L-fucosidase-deficient, EBV-immortalized lymphoid cells from a fucosidosis patient. Conjugates were effectively bound and internalized by the cells in a lactose inhibitable manner. Internalization of conjugate resulted in the reduced accumulation of alpha-L-fucosyl-N-acetylglucosaminylasparagine, a glycopeptide that accumulates in cells of fucosidosis patients, to levels found in lymphoid cells from a healthy individual. Thus, galaptin-alpha-L-fucosidase conjugates may be useful for enzyme replacement therapy of fucosidosis. The concept of using galaptin as a transport vehicle may be applied to the delivery of other compounds to cells bearing galaptin receptors.

• Authors: H J Allen, H Ahmed, R A DiCioccio
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 172 Issue 1 Pg. 335-40 (Oct 15 1990) ISSN: 0006-291X [Print] United States
• PMID: 2222477 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Carrier Proteins
• Galectins
• Hemagglutinins
• alpha-L-Fucosidase

Topics

• B-Lymphocytes (drug effects, metabolism)
• Carrier Proteins (metabolism)
• Cell Line
• Fucosidosis (metabolism)
• Galectins
• Hemagglutinins (metabolism, pharmacology)
• Humans
• Kinetics
• Reference Values
• alpha-L-Fucosidase (metabolism, pharmacology)