More than 90% of
malignant neoplasms of the oral cavity are
squamous carcinomas of oral mucosa and most are conventional type. This study included 60 cases of oral
squamous carcinoma processed by usual histological technique and stained with
Hematoxylin-
Eosin and
Alcian Blue-
Safranin (
Alcian Blue powder from Sigma Aldrich, code A5268-25G) and diagnosed in the Department of Pathology, University of Medicine and Pharmacy of Craiova. Double immunohistochemical (CD105-tryptase, VEGF-, VEGFR1-, VEGFR2-tryptase) or combined histochemical and immunohistochemical reactions (VEGF÷ VEGFR1÷ VEGFR2÷Alcian Blue-
Safranin) followed the particular morphological aspects of the mast cells, their relation with the blood vessels and the overlap signal of
tryptase and
Alcian Blue-Safranin÷VEGF and its R1 and R2 receptors in mast cells. Immunostaining for
VEGF and its R1 and R2 receptors was present both in
tumor cells and mast cells. Double immunohistochemical-histochemical reactions allowed us angiogenic profiling the mast cells. The signal overlap was present for
VEGF-, VEGFR1-, VEGFR2-tryptase intratumoral and
tumor invasion front mast cells. Student's t-test for comparison of intratumoral and the invasion front MDM showed highly significant value (p=3.23 E-08). VEGF÷ VEGFR1÷ VEGFR2÷Alcian Blue-
Safranin revealed particular morphological aspects of mast cells, in with different morphology, shapes, sizes and degrees of degranulation. Statistical analysis showed a linear correlation between MDM and MVD inside the
tumor (Pearson coefficient =0.47) and a weak linear correlation at the front of invasion (Pearson coefficient =0.19). This study has highlighted the importance of mast cells in the
tumor growth of the oral
squamous carcinomas, especially in terms of their proangiogenic profile (expression of
VEGF and its R1 and R2 receptors). In addition, their quantification as MDM makes this parameter a useful prognostic marker.