Abstract |
Due to the poor immunogenicity of subunit protein antigens, there is a need to use adjuvants in order to generate effective immune responses. Basic fibroblast growth factor (bFGF) is one of the best characterized pro-angiogenic cytokine and is a candidate target for anticancer therapy. We used truncated bFGF (tbFGF) combined with engineered pVAX-nCpG as novel adjuvant to immunize mice in order to inhibit tumor angiogenesis and suppress tumor growth. In our study, the results demonstrated that the mice immunized with tbFGF-alum-pVAX-8CpG produced a better tumor-suppression effect compared with the other groups, apart from the group treated with tbFGF- alum-CpG. In addition, the function of immune modulation of pVAX-8CpG was similar to CpG ODNs. The vaccine composed of tbFGF, alum and pVAX-8CpG effectively inhibited tumor angiogenesis and induced strong antitumor immune responses. The antitumor activity induced by the vaccine tbFGF-alum-pVAX-8CpG was not only associated with the antigen-specific antibody, but also with the killing activity of cytotoxic cells. This indicates that alum-pVAX-8CpG may be an innovative adjuvant for cancer vaccines.
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Authors | Meng Li, Hua Shan Shi, Hai Long Zhang, Zi Chao Luo, Yang Wan, Lian Lu, Shun-Tao Luo, Li Yang |
Journal | Molecular medicine reports
(Mol Med Rep)
Vol. 5
Issue 3
Pg. 625-30
(03 2012)
ISSN: 1791-3004 [Electronic] Greece |
PMID | 22200843
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Alum Compounds
- Antibodies, Neutralizing
- Cancer Vaccines
- Recombinant Proteins
- Fibroblast Growth Factor 2
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Alum Compounds
(therapeutic use)
- Animals
- Antibodies, Neutralizing
(immunology, metabolism)
- Cancer Vaccines
(immunology, therapeutic use)
- Carcinoma, Lewis Lung
(immunology, therapy)
- Female
- Fibroblast Growth Factor 2
(genetics, immunology, metabolism)
- Mice
- Mice, Inbred C57BL
- Neovascularization, Pathologic
- Plasmids
(metabolism, therapeutic use)
- Recombinant Proteins
(genetics, metabolism, therapeutic use)
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