HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

bFGF peptide combined with the pVAX-8CpG plasmid as adjuvant is a novel anticancer vaccine inducing effective immune responses against Lewis lung carcinoma.

Abstract
Due to the poor immunogenicity of subunit protein antigens, there is a need to use adjuvants in order to generate effective immune responses. Basic fibroblast growth factor (bFGF) is one of the best characterized pro-angiogenic cytokine and is a candidate target for anticancer therapy. We used truncated bFGF (tbFGF) combined with engineered pVAX-nCpG as novel adjuvant to immunize mice in order to inhibit tumor angiogenesis and suppress tumor growth. In our study, the results demonstrated that the mice immunized with tbFGF-alum-pVAX-8CpG produced a better tumor-suppression effect compared with the other groups, apart from the group treated with tbFGF-alum-CpG. In addition, the function of immune modulation of pVAX-8CpG was similar to CpG ODNs. The vaccine composed of tbFGF, alum and pVAX-8CpG effectively inhibited tumor angiogenesis and induced strong antitumor immune responses. The antitumor activity induced by the vaccine tbFGF-alum-pVAX-8CpG was not only associated with the antigen-specific antibody, but also with the killing activity of cytotoxic cells. This indicates that alum-pVAX-8CpG may be an innovative adjuvant for cancer vaccines.
AuthorsMeng Li, Hua Shan Shi, Hai Long Zhang, Zi Chao Luo, Yang Wan, Lian Lu, Shun-Tao Luo, Li Yang
JournalMolecular medicine reports (Mol Med Rep) Vol. 5 Issue 3 Pg. 625-30 (03 2012) ISSN: 1791-3004 [Electronic] Greece
PMID22200843 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adjuvants, Immunologic
  • Alum Compounds
  • Antibodies, Neutralizing
  • Cancer Vaccines
  • Recombinant Proteins
  • Fibroblast Growth Factor 2
Topics
  • Adjuvants, Immunologic (administration & dosage)
  • Alum Compounds (therapeutic use)
  • Animals
  • Antibodies, Neutralizing (immunology, metabolism)
  • Cancer Vaccines (immunology, therapeutic use)
  • Carcinoma, Lewis Lung (immunology, therapy)
  • Female
  • Fibroblast Growth Factor 2 (genetics, immunology, metabolism)
  • Mice
  • Mice, Inbred C57BL
  • Neovascularization, Pathologic
  • Plasmids (metabolism, therapeutic use)
  • Recombinant Proteins (genetics, metabolism, therapeutic use)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: