The indolic diet-derivative, 3,3'-diindolylmethane, induced apoptosis in human colon cancer cells through upregulation of NDRG1.

Abstract

N-myc downstream regulated gene-1 participates in carcinogenesis, angiogenesis, metastases, and anticancer drug resistance. In the present study, we analyzed the expression pattern of N-myc downstream regulated gene-1 following treatment of human colonic cancer cell lines; HCT-116 (well differentiated with wild-type p53 gene) and Colo-320 (poorly differentiated with mutant p53 gene), with 3,3'-diindolylmethane, a well-established proapoptotic agent product derived from indole-3-carbinol. Treatment of Colo-320 and HCT-116 with 3,3'-diindolylmethane disclosed inhibition of cell viability in a dose-dependent manner, mediated through apoptosis induction. The increased expression of N-myc downstream regulated gene-1 was detected only in poorly differentiated colon cancer cells, Colo-320 cell line. Our results suggest that N-myc downstream regulated gene-1 expression is enhanced by 3,3'-diindolylmethane in poorly differentiated cells and followed by induction of apoptosis. 3,3'-diindolylmethane induced apoptosis may represent a new regulator of N-myc downstream regulated gene-1 in poorly differentiated colonic cancer cells.

• Authors: A Lerner, M Grafi-Cohen, T Napso, N Azzam, F Fares
• Journal: Journal of biomedicine & biotechnology (J Biomed Biotechnol) Vol. 2012 Pg. 256178 ( 2012) ISSN: 1110-7251 [Electronic] United States
• PMID: 22187533 (Publication Type: Journal Article)

Chemical References

• Anticarcinogenic Agents
• Cell Cycle Proteins
• Indoles
• Intracellular Signaling Peptides and Proteins
• N-myc downstream-regulated gene 1 protein
• 3,3'-diindolylmethane

Topics

• Anticarcinogenic Agents (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Cycle Proteins (biosynthesis, genetics, metabolism)
• Cell Differentiation (drug effects)
• Cell Survival (drug effects)
• Colonic Neoplasms (drug therapy, metabolism, pathology)
• Gene Expression Regulation, Neoplastic (drug effects)
• HCT116 Cells
• Humans
• Indoles (pharmacology)
• Intracellular Signaling Peptides and Proteins (genetics, metabolism)
• Up-Regulation (drug effects)