Preventive effects of green tea (Camellia sinensis var. assamica) on diabetic nephropathy.

Abstract

PURPOSE:

This study aimed to evaluate the preventive effects of Camellia sinensis var. assamica (CSVA) on diabetic nephropathy in in vitro and in vivo models.

MATERIALS AND METHODS:

MDCK cells were incubated with 1 mM of oxalate with or without different concentrations of CSVA, then MTT and malondialdehyde (MDA) assays were performed to investigate the preventive effects of CSVA on oxalate-induced cytotoxicity and oxidative stress. Thirty male db/db mice were divided into three groups. Group 1 were fed AIN-93G ad libitum; group 2 were fed AIN-93G mixed with 10% fermented CSVA ad libitum; group 3 were fed AIN-93G mixed with 10% non-fermented CSVA ad libitum. The mice were sacrificed 14 weeks later, and the serum glucose level, 24-hour urine chemistry, and morphological changes in the kidneys were examined.

RESULTS:

As CSVA concentrations increased, viable MDCK cells increased in concentration. MDA production decreased over time in the CSVA treated group. The creatinine clearance of group 3 was lower than those of groups 1 and 2. The amount of urine microalbumin and protein in group 1 were higher than those in groups 2 and 3. Also, more glomerulus basement membrane foot processes were preserved in groups 2 and 3.

CONCLUSION:

In conclusion, CSVA has beneficial preventive tendencies towards diabetic nephropathy in both in vitro and in vivo models.

• Authors: Min-Yong Kang, Yong Hyun Park, Bong Sub Kim, Su Yeon Seo, Byong Chang Jeong, Jung-In Kim, Hyeon Hoe Kim
• Journal: Yonsei medical journal (Yonsei Med J) Vol. 53 Issue 1 Pg. 138-44 (Jan 2012) ISSN: 1976-2437 [Electronic] Korea (South)
• PMID: 22187244 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Extracts
• Tea

Topics

• Animals
• Camellia sinensis (chemistry)
• Cell Line
• Cell Survival (drug effects)
• Diabetes Mellitus, Type 2 (complications)
• Diabetic Nephropathies (drug therapy, prevention & control)
• Disease Models, Animal
• Dogs
• Kidney (cytology, drug effects)
• Male
• Mice
• Mice, Mutant Strains
• Plant Extracts (pharmacology)
• Tea (chemistry)