Abstract | UNLABELLED: The aim of this study was to determine adhesion and colonization of bacteria on the surface of originally synthesized glass-ceramic biomaterials and their effect on inflammation reactions in tissues surrounding the implant. MATERIALS AND METHODS:
Biomaterial discs were contaminated with bacterial suspensions of 10, 10(2), and 10(3) colony forming units (CFU)/mL (P. aeruginosa ATCC 27853 and S. epidermidis ATCC 12228), and after 2 hours of cultivation, the intensity of bacterial adhesion was determined. For in vivo tests, the samples were contaminated with 102 and 103 CFU/mL cultivated at 37°C for 2 h to ensure bacterial adhesion. Contaminated biomaterial samples were implanted in the interscapular area of chinchilla rabbits for 2 and 4 weeks. The biomaterials were removed, and using plate count and sonification methods, bacterial colonization on the surface of biomaterials was determined. Moreover, the expression of TNF-α, β- defensin 2, and IL-10 in the surrounding tissues was assessed by using immunohistochemistry methods. RESULTS: P. aeruginosa more intensively colonized biomaterials in the in vivo study as compared with S. epidermidis. Il-10 is a regulatory cytokine, which reduces the intensity of inflammatory cell activity, thus reducing nonspecific resistance of the organism. CONCLUSIONS: The expression of TNF-α and IL-10 was not affected by short (2 and 4 weeks) biomaterial implantation. Pronounced cytokine expression in tissues around implanted biomaterials contaminated with P. aeruginosa was observed.
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Authors | Aigars Reinis, Māra Pilmane, Agnese Stunda, Jānis Vētra, Juta Kroiča, Dagnija Rostoka, Girts Salms, Antons Vostroilovs, Aleksejs Dons, Līga Bērziņa-Cimdiņa |
Journal | Medicina (Kaunas, Lithuania)
(Medicina (Kaunas))
Vol. 47
Issue 10
Pg. 560-5
( 2011)
ISSN: 1648-9144 [Electronic] Switzerland |
PMID | 22186120
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biocompatible Materials
- Tumor Necrosis Factor-alpha
- beta-Defensins
- Interleukin-10
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Topics |
- Animals
- Bacterial Adhesion
- Biocompatible Materials
(chemical synthesis, chemistry)
- Ceramics
(chemistry)
- Glass
(chemistry)
- Interleukin-10
(biosynthesis)
- Prostheses and Implants
(microbiology)
- Prosthesis Implantation
- Pseudomonas aeruginosa
(physiology)
- Rabbits
- Staphylococcus epidermidis
(physiology)
- Surface Properties
- Tumor Necrosis Factor-alpha
(biosynthesis)
- beta-Defensins
(biosynthesis)
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