[Application of multiplex nested RT-PCR for fast detection of PDGFRα fusion gene in myeloproliferative neoplasms].

Abstract

This study was aimed to explore the applicable value of multiplex nested reverse transcription-polymerase chain reaction (multiplex nested RT-PCR)for the detection of platelet-derived growth factor receptor alpha (PDGFRα) fusion gene in myeloproliferative neoplasms (MPN). Bone marrow or peripheral blood samples from 146 patients with MPN were analyzed by using a novel multiplex nested RT-PCR. The result showed that PDGFRα fusion gene was found in 6 out of the 146 bone marrow or peripheral blood samples, the positive rate was 4.11%, 4 from the 6 patients received treatment with imatinib and showed therapeutic effect. It is concluded that the multiplex nested RT-PCR has a series of advantages such as high sensitivity, specificity, and time-saving, and can be applied for determination of the molecular type of MPN, and also for the diagnosis and therapy of MPN.

• Authors: Meng-Meng Jiang, Min-Hang Zhou, Li Gao, Yi Ding, Yuan-Yuan Xu, Li-Li Wang, Yu Jing, Quan-Shun Wang, Li Yu
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 19 Issue 6 Pg. 1447-9 (Dec 2011) ISSN: 1009-2137 [Print] China
• PMID: 22169301 (Publication Type: English Abstract, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Receptor, Platelet-Derived Growth Factor alpha

Topics

• Bone Marrow Neoplasms (diagnosis, genetics)
• Gene Fusion
• Humans
• Multiplex Polymerase Chain Reaction
• Myeloproliferative Disorders (diagnosis, genetics)
• Receptor, Platelet-Derived Growth Factor alpha (genetics)
• Reverse Transcriptase Polymerase Chain Reaction (methods)