Abstract | OBJECTIVE: METHODS: The expression product of SjLAP was identified by Western blot and further purified by using nickel column. The IgG levels in the response to SjLAP in murine and porcine sera were detected by ELISA at different time points after the infection of S. japonicum. RESULTS: SjLAP expressed by E. coli was recognized by anti-his monoclonal antibody and S. japonicum-infected mice sera by Western blot. The results of ELISA showed that IgG responses to SjLAP rose gradually and reached the peak at 4 weeks post- infection for pigs (P1) and 6 weeks post- infection for mice (P2). With the appearance of a large number of eggs in the tissue, SjLAP-specific IgG levels were significantly down-regulated ( P1 = 0. 0004, P2 = 0. 0001). CONCLUSION:
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Authors | Xia Zhang, Ya-Nan Gao, Min Hou, Lin Chen, Min-Jun Ji, Guan-Ling Wu |
Journal | Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control
(Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi)
Vol. 23
Issue 2
Pg. 163-7
(Apr 2011)
ISSN: 1005-6661 [Print] China |
PMID | 22164617
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Helminth
- Helminth Proteins
- Immunoglobulin G
- Leucyl Aminopeptidase
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Topics |
- Animals
- Antibodies, Helminth
(immunology)
- Antibody Formation
- Female
- Helminth Proteins
(genetics, immunology)
- Humans
- Immunoglobulin G
(immunology)
- Leucyl Aminopeptidase
(genetics, immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Schistosoma japonicum
(enzymology, growth & development, immunology)
- Schistosomiasis japonica
(blood, diagnosis, immunology, parasitology)
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