[Detection of urine antibodies to Schistosoma japonicum by magnetic particle affinity immunoassay].

Abstract

OBJECTIVE:

To explore a non-invasive method for detection of urine antibodies to Schistosoma japonicum.

METHODS:

The urine antibodies to S. japonicum were detected by magnetic particle affinity immunoassay (MPAIA) in 158 cases of schistosomiasis japonica and 100 health persons, and their serum antibodies to S. japonicum were also detected at the same time.

RESULTS:

The sample of urine by MPAIA was 10 microl original urine without any special treatment. The positive rate of urine and serum were 48.10% (76/158)and 88.61% (140/158), respectively. There was difference between the performance of two methods (chi2 = 60.24, P < 0.05). However, both of their specificity were 100% (100/100).

CONCLUSION:

MPAIA is viable for detection of urine antibodies to S. japonicum, but its sensitivity should be improved.

• Authors: Chao-Wei Li, Jin Huang, Lan-Ying Wei, Chang-Fu Jiang
• Journal: Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control (Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi) Vol. 23 Issue 3 Pg. 327-9 (Jun 2011) ISSN: 1005-6661 [Print] China
• PMID: 22164507 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Helminth

Topics

• Animals
• Antibodies, Helminth (blood, urine)
• Humans
• Immunoassay (instrumentation, methods)
• Magnetics (methods)
• Schistosoma japonicum (immunology, isolation & purification)
• Schistosomiasis japonica (blood, diagnosis, urine)
• Sensitivity and Specificity