Abstract | BACKGROUND: Cruciferous vegetable constituent phenethyl isothiocyanate ( PEITC) causes apoptosis in prostate cancer cells through mechanisms not fully understood. The present study was designed to determine the role of inhibitor of apoptosis (IAP) family proteins in PEITC-induced apoptosis induction. METHODS: Effect of PEITC treatment on protein and mRNA expression of IAP in cells was determined by Western blotting and reverse transcription PCR, respectively. Immunohistochemistry was performed to determine the in vivo effect of PEITC administration on X-linked IAP (XIAP) and Survivin protein expression. Overexpression of desired protein was achieved by transient transfection. Cell viability was determined by trypan blue dye exclusion assay, whereas apoptosis was quantified by measurement of histone-associated DNA fragment release into the cytosol. Transwell chamber assay was used to determine cell migration. RESULTS: CONCLUSIONS: The present study demonstrates that cellular responses to PEITC, including apoptosis induction and inhibition of cell migration, in prostate cancer cells are mediated by downregulation of XIAP and/or Survivin, which may serve as valid biomarkers of PEITC response in future clinical investigations.
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Authors | Kozue Sakao, Sudhakar Desineni, Eun-Ryeong Hahm, Shivendra V Singh |
Journal | The Prostate
(Prostate)
Vol. 72
Issue 10
Pg. 1104-16
(Jul 01 2012)
ISSN: 1097-0045 [Electronic] United States |
PMID | 22161756
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2011 Wiley Periodicals, Inc. |
Chemical References |
- Inhibitor of Apoptosis Proteins
- Isothiocyanates
- phenethyl isothiocyanate
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Topics |
- Animals
- Cell Line, Tumor
- Gene Expression Regulation, Neoplastic
- Humans
- Inhibitor of Apoptosis Proteins
(antagonists & inhibitors, biosynthesis)
- Isothiocyanates
(pharmacology)
- Male
- Mice
- Mice, Transgenic
- Prostatic Neoplasms
(metabolism, pathology)
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