Abstract |
Multiple reports have claimed that low-dose orally administered interferon (IFN)-α is beneficial in the treatment of many infectious diseases and provides a viable alternative to high-dose intramuscular treatment. However, research is needed on how to express IFN stably in the gut. Bifidobacterium may be a suitable carrier for human gene expression and secretion in the intestinal tract for the treatment of gastrointestinal diseases. We reported previously that Bifidobacterium longum can be used as a novel oral delivery of IFN-α. IFN-transformed B. longum can exert an immunostimulatory role in mice; however the answer to whether this recombinant B. longum can be used to treat virus infection still remains elusive. Here, we investigated the efficacy of IFN-transformed B. longum administered orally on coxsackie virus B3 (CVB3)-induced myocarditis in BALB/c mice. Our data indicated that oral administration of IFN-transformed B. longum for 2 weeks after virus infection reduced significantly the severity of virus-induced myocarditis, markedly down regulated virus titers in the heart, and induced a T helper 1 cell pattern in the spleen and heart compared with controls. Oral administration of the IFN-transformed B. longum, therefore, may play a potential role in the treatment of CVB3-induced myocarditis.
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Authors | Zhijian Yu, Zhen Huang, Chongwen Shao, Yuanjian Huang, Fan Zhang, Jin Yang, Lili Deng, Zhongming Zeng, Qiwen Deng, Weiseng Zeng |
Journal | Virology journal
(Virol J)
Vol. 8
Pg. 525
(Dec 08 2011)
ISSN: 1743-422X [Electronic] England |
PMID | 22151967
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interferon-alpha
- Recombinant Fusion Proteins
- Recombinant Proteins
- Polyethylene Glycols
- peginterferon alfa-2a
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Topics |
- Animals
- Bifidobacterium
(genetics, immunology)
- Coxsackievirus Infections
(immunology, prevention & control, virology)
- Enterovirus
(drug effects, immunology)
- Gene Transfer Techniques
- Humans
- Interferon-alpha
(genetics, immunology, metabolism)
- Intestines
(immunology, microbiology)
- Male
- Mice
- Mice, Inbred BALB C
- Myocarditis
(immunology, prevention & control, virology)
- Polyethylene Glycols
- Promoter Regions, Genetic
- Recombinant Fusion Proteins
(genetics, immunology, metabolism)
- Recombinant Proteins
(genetics, immunology, metabolism)
- Spleen
(immunology, microbiology)
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