Effect of polyethylene glycol in pig intestinal allotransplantation without immunosuppression.

Abstract

OBJECTIVES:

We evaluated whether IGL-1, a graft preservation solution containing polyethylene glycol, improves the outcome of small bowel grafts in comparison to the University of Wisconsin (UW) solution in a pig allotransplantation model.

MATERIALS AND METHODS:

Seventeen pigs were randomly allocated to group 1 (n = 10; intestinal allotransplantation with IGL-1) and group 2 (n = 7; allotransplantation with UW). Pigs received no immunosuppression and were sacrificed on postoperative d (POD) 8. Intestinal specimens were obtained from the animal immediately before cold flushing (T0), 2 h after graft reperfusion (T1), and at sacrifice (T2).

RESULTS:

Survival rate to POD 8 was 50% in group 1 compared with 16% in group 2 (P < 0.05); 62% of pigs in group 1 did not present any acute cellular rejection (ACR) compared to 16% in group 2 (P < 0.05). Severe ACR rate was 25% in group 1 and 66% in group 2 (P < 0.05). iNOS activity and intestinal caspase 3 levels increased significantly between T0 and T1 in group 1 compared to group 2 (P < 0.05). Cell necrosis increased significantly between TO and T1 in group 2 compared with group 1 (P < 0.05) whereas cell apoptosis was significantly higher at T1 compared with T0 in group 1 in comparison to group 2.

CONCLUSIONS:

Our results show that IGL-1 improves intestinal graft viability as compared to UW solution, possibly by reducing graft immunogenicity and by favoring intestinal epithelial repair.

• Authors: Thierry Yandza, Michel Tauc, Danielle Canioni, Claire Rogel-Gaillard, Ghislaine Bernard, Alain Bernard, Jean Gugenheim
• Journal: The Journal of surgical research (J Surg Res) Vol. 176 Issue 2 Pg. 621-8 (Aug 2012) ISSN: 1095-8673 [Electronic] United States
• PMID: 22137987 (Publication Type: Comparative Study, Journal Article)
• Copyright: Copyright © 2012 Elsevier Inc. All rights reserved.

Chemical References

• IGL-1 solution
• Insulin
• Organ Preservation Solutions
• University of Wisconsin-lactobionate solution
• Polyethylene Glycols
• Allopurinol
• Caspase 3
• Glutathione
• Adenosine
• Raffinose

Topics

• Acute Disease
• Adenosine (pharmacology)
• Allopurinol (pharmacology)
• Animals
• Apoptosis (immunology)
• Caspase 3 (metabolism)
• Female
• Glutathione (pharmacology)
• Graft Rejection (immunology, mortality, prevention & control)
• Graft Survival (drug effects, immunology)
• Immunosuppression Therapy
• Insulin (pharmacology)
• Intestinal Mucosa (immunology, pathology)
• Intestine, Small (immunology, pathology, transplantation)
• Organ Preservation Solutions (pharmacology)
• Polyethylene Glycols (pharmacology)
• Raffinose (pharmacology)
• Reperfusion Injury (immunology, mortality, prevention & control)
• Sus scrofa
• Transplantation, Homologous