HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Antifibrotic effects of protocatechuic aldehyde on experimental liver fibrosis.

AbstractBACKGROUND:
Recent studies have demonstrated that transforming growth factor-β1 (TGF-β1) and connective transforming growth factor (CTGF) are associated with the pathophysiology of liver fibrosis. We isolated protocatechuic aldehyde, the major degradation of phenolic acids.
OBJECTIVE:
This study was carried out to investigate the potential antifibrotic effect of Protocatechuic aldehyde (PA) on experimental liver fibrosis in vitro and in vivo, and to explore its possible mechanism.
MATERIALS AND METHODS:
Cell proliferation was determined. Type I collagen, type III collagen, transforming growth factor-β1 (TGF-β1) and connective transforming growth factor (CTGF) were measured by ELISA kits in TNF-α stimulated HSCs. In the carbon tetrachloride (CCL(4))-induced rat liver fibrosis model, liver fibrosis grade and histopathological changes were evaluated, and biochemical indicators were determined. Furthermore, immunostaining and Western blot analysis were used to detect hepatic TGF-β1 and CTGF expression in liver tissue.
RESULTS:
Overall, our results indicated that PA inhibits HSCs proliferation, inhibits the levels of TGF-β1, CTGF, type I collagen and type III collagen in TNF-α stimulated HSCs. Treatment of PA causes a significant reduction in fibrosis grade, ameliorates biochemical indicators and histopathological morphology, and reduces liver TGF-β1 and CTGF expression in rat model of CCL(4)-induced liver fibrosis.
CONCLUSION:
These findings suggest that PA has potentially conferring antifibrogenic effects.
AuthorsChunmei Li, Wanglin Jiang, Haibo Zhu, Jian Hou
JournalPharmaceutical biology (Pharm Biol) Vol. 50 Issue 4 Pg. 413-9 (Apr 2012) ISSN: 1744-5116 [Electronic] England
PMID22129045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Retracted Publication)
Chemical References
  • Benzaldehydes
  • CCN2 protein, rat
  • Catechols
  • Collagen Type I
  • Collagen Type III
  • Laminin
  • Protective Agents
  • Tgfb1 protein, rat
  • Transforming Growth Factor beta1
  • Tumor Necrosis Factor-alpha
  • Connective Tissue Growth Factor
  • protocatechualdehyde
  • Hyaluronic Acid
  • Carbon Tetrachloride
Topics
  • Animals
  • Benzaldehydes (pharmacology)
  • Blotting, Western
  • Carbon Tetrachloride
  • Catechols (pharmacology)
  • Cell Proliferation (drug effects)
  • Cells, Cultured
  • Collagen Type I (metabolism)
  • Collagen Type III (metabolism)
  • Connective Tissue Growth Factor (metabolism)
  • Cytoprotection
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Enzyme-Linked Immunosorbent Assay
  • Hepatic Stellate Cells (drug effects, metabolism, pathology)
  • Hyaluronic Acid (metabolism)
  • Immunohistochemistry
  • Laminin (metabolism)
  • Liver (drug effects, metabolism, pathology)
  • Liver Cirrhosis (chemically induced, metabolism, pathology, prevention & control)
  • Male
  • Protective Agents (pharmacology)
  • Rats
  • Rats, Sprague-Dawley
  • Transforming Growth Factor beta1 (metabolism)
  • Tumor Necrosis Factor-alpha (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: