Presence of glucose in dialyzing fluid and synthesis of selected lipoxygenase-derived eicosanoids during hemodialysis.

Abstract

PURPOSE:

Active metabolites of arachidonic acid (AA), eicosanoids, are exerting a significant influence on renal homeostasis. In our recent paper, we demonstrated that high levels of lipoxygenase (LOX)-derived AA metabolites, hydroxyeicosatetraenoic acids (HETEs), unfavorably influence post-transplant function of kidney allografts. Hence, in this study, we wanted (1) to analyze 5-, 12- and 15-HETE levels in non-diabetic chronic kidney disease (CKD) patients, already undergoing regular hemodialysis treatment, and determine factors that may influence these eicosanoids' generation, as well as, (2) to verify whether application of glucose-containing, instead of glucose-free, dialyzing fluids may be beneficial for the limitation of 5-, 12- and 15-HETE synthesis during a single hemodialysis session.

METHODS:

Twenty-four healthy individuals and 50 CKD patients undergoing regular hemodialysis treatment were included in the study. CKD patients were divided into two subgroups depending on presence/absence of glucose in dialyzing fluid. LOX-derived HETEs were measured using liquid chromatography.

RESULTS:

Results demonstrated higher levels of examined eicosanoids in CKD patients (P<0.05 for all). Older age and higher C-reactive protein levels were associated with HETEs concentrations. Presence of glucose in dialyzing fluid significantly diminished the increase in 5- and 12-HETE synthesis (411.24% vs. 107.29%, P<0.006; 301.70% vs. 98.21%, P<0.0008, respectively), however, it did not influence 15-HETE generation (156.98% vs. 135.24%, P=0.26).

CONCLUSIONS:

Non-diabetic CKD patients have higher levels of LOX-derived HETEs, which are associated mainly with age and intensified inflammatory process. The presence of glucose in the dialysate is associated with a reduced synthesis of selected LOX-derived HETEs in these patients; hence, we speculate that the application of such a simple hemodialysis fluid modification may have a favorable influence on post-transplant outcomes.

• Authors: Barbara Dołęgowska, Wojciech Błogowski, Joanna Stępniewska, Krzysztof Safranow, Katarzyna Jakubowska, Maria Olszewska
• Journal: International urology and nephrology (Int Urol Nephrol) Vol. 44 Issue 6 Pg. 1799-804 (Dec 2012) ISSN: 1573-2584 [Electronic] Netherlands
• PMID: 22127406 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hemodialysis Solutions
• Hydroxyeicosatetraenoic Acids
• Lipoxygenase
• Glucose

Topics

• Female
• Glucose (analysis)
• Hemodialysis Solutions (chemistry)
• Humans
• Hydroxyeicosatetraenoic Acids (biosynthesis)
• Lipoxygenase (metabolism)
• Male
• Middle Aged
• Renal Dialysis
• Renal Insufficiency, Chronic (metabolism, therapy)