[Immunogenicity of attenuated Salmonella choleraesuis vaccine strain expressing immunogenic genes of Mycoplasma hyopneumoniae in mice].

Abstract

OBJECTIVE:

The study was carried out to construct and characterize Salmonella choleraesuis vaccine strain expressing immunogenic genes of Mycoplasma hyopneumoniae and to test its immunogenicity in mice.

METHOD:

We made p36, p46, p65 and p97R1-Nrdf, the main immunogenic genes of Mycoplasma hyopneumoniae, to insert into the prokaryotic expression plasmid pYA3493. Then these recombinant plasmids and pYA3493 were electroporated into C500 asd-mutant, resulting in the recombinant Salmonella choleraesuis vaccine strains C36 (pYA-36), C46 (pYA-46), C65 (pYA-65), C97R1-Nrdf(pYA-97R1-Nrdf) and CpYA(pYA3493). We characterized these recombinant Salmonella choleraesuis vaccine strains and tested the immunogenicity in mice by intramuscular injection or orally immunized.

RESULT:

The results of the immunogenicity in mice indicated that the group orally immunized with C36, C46, C65, C97R1-Nrdf showed significantly higher Mycoplasma pneumoniae antibody than both the group orally immunized with C36, C46, C65 and the group intramuscular injected with the Mycoplasma hyopneumoniae bacterin (M + PAC) (P < 0.01). The group intramuscular injected with C36, C46, C65 showed higher IFN-gamma production than the group injected with the Mycoplasma hyopneumoniae bacterin (M + PAC) (P < 0.05), but there was no significant difference between the group orally immunized with C36, C46, C65 and the group orally immunized with C36, C46, C65, C97R1-Nrdf (P > 0.05). The highest level of IL-4 was found in the group orally immunized with C36, C46, C65; higher levels of IL-4 was observed in the group orally immunized with C36, C46, C65, C97R1-Nrdf than the group injected with the Mycoplasma hyopneumoniae bacterin (M + PAC); and the lowest IL-4 level was found in the group injected with C36, C46, C65. There were no significant differences among them (P > 0.05). The Mycoplasma pneumoniae antibody, IFN-gamma or IL-4 production of the each group was obviously higher than the control group (P < 0.01).

CONCLUSION:

The attenuated Salmonella choleraesuis vaccine strain expressing immunogenic genes of Mycoplasma hyopneumoniae which has immunogenicity in mice especially by intramuscular injection could probably serve as a vaccine against mycoplasmal pneumonia of swine.

• Authors: Fengying Ma, Haoyong Zou, Qigai He
• Journal: Wei sheng wu xue bao = Acta microbiologica Sinica (Wei Sheng Wu Xue Bao) Vol. 51 Issue 9 Pg. 1270-7 (Sep 2011) ISSN: 0001-6209 [Print] China
• PMID: 22126084 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Bacterial Vaccines
• Vaccines, Attenuated
• Interleukin-4
• Interferon-gamma

Topics

• Animals
• Antibodies, Bacterial (blood)
• Bacterial Vaccines (genetics, immunology)
• Genetic Vectors (genetics)
• Immunization
• Interferon-gamma (blood)
• Interleukin-4 (blood)
• Lymphocytes (immunology)
• Mice
• Mice, Inbred BALB C
• Mycoplasma hyopneumoniae (genetics, immunology)
• Salmonella (genetics, immunology)
• Spleen (immunology)
• Vaccines, Attenuated (genetics, immunology)