HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Proteomic analysis of the nucleus accumbens in rhesus monkeys of morphine dependence and withdrawal intervention.

Abstract
It has been known that the reinforcing effects and long-term consequences of morphine are closely associated with nucleus accumbens (NAc) in the brain, a key region of the mesolimbic dopamine pathway. However, the proteins involved in neuroadaptive processes and withdrawal symptom in primates of morphine dependence have not been well explored. In the present study, we performed proteomes in the NAc of rhesus monkeys of morphine dependence and withdrawal intervention with clonidine or methadone. Two-dimensional electrophoresis was used to compare changes in cytosolic protein abundance in the NAc. We found a total of 46 proteins differentially expressed, which were further identified by mass spectrometry analysis. The identified proteins can be classified into 6 classes: metabolism and mitochondrial function, synaptic transmission, cytoskeletal proteins, oxidative stress, signal transduction and protein synthesis and degradation. Importantly, we discovered 14 proteins were significantly but similarly altered after withdrawal therapy with clonidine or methadone, revealing potential pharmacological strategies or targets for the treatment of morphine addiction. Our study provides a comprehensive understanding of the neuropathophysiology associated with morphine addiction and withdrawal therapy in primate, which is helpful for the development of opiate withdrawal pharmacotherapies.
AuthorsQian Bu, Yanzhu Yang, Guangyan Yan, Zhengtao Hu, Chunyan Hu, Jiachuan Duan, Lei Lv, Jiaqing Zhou, Jinxuan Zhao, Xue Shao, Yi Deng, Yan Li, Hongyu Li, Ruiming Zhu, Yinglan Zhao, Xiaobo Cen
JournalJournal of proteomics (J Proteomics) Vol. 75 Issue 4 Pg. 1330-42 (Feb 02 2012) ISSN: 1876-7737 [Electronic] Netherlands
PMID22123079 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2011 Elsevier B.V. All rights reserved.
Chemical References
  • Morphine
Topics
  • Animals
  • Behavior, Animal
  • Body Weight
  • Cytosol (metabolism)
  • Electrophoresis, Gel, Two-Dimensional (methods)
  • Gene Expression Regulation
  • Macaca mulatta
  • Male
  • Mass Spectrometry (methods)
  • Morphine (metabolism)
  • Morphine Dependence (metabolism)
  • Nucleus Accumbens (metabolism)
  • Proteomics (methods)
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)
  • Substance Withdrawal Syndrome (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: