Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens.

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

• Authors: Leisha J Richardson, Mirjam Kaestli, Mark Mayo, Jolene R Bowers, Apichai Tuanyok, Jim Schupp, David Engelthaler, David M Wagner, Paul S Keim, Bart J Currie
• Journal: Journal of microbiological methods (J Microbiol Methods) Vol. 88 Issue 1 Pg. 179-81 (Jan 2012) ISSN: 1872-8359 [Electronic] Netherlands
• PMID: 22108495 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• DNA, Bacterial

Topics

• Burkholderia pseudomallei (chemistry, genetics, isolation & purification)
• DNA, Bacterial (blood, genetics, isolation & purification)
• Humans
• Melioidosis (diagnosis, microbiology)
• Pathology, Molecular (methods)
• Real-Time Polymerase Chain Reaction