Abstract | BACKGROUND: Using imaging mass spectrometry (IMS), we investigated the cerebral protective effect of an artificial cerebrospinal fluid (CSF), ARTCEREB (Artcereb, Otsuka Pharmaceutical Factory, Inc., Tokushima, Japan), as an irrigation and perfusion solution for neurosurgical procedures in a rat craniotomy model. METHODS:
Wounds created in the rat cerebral cortex were continuously irrigated with Artcereb, normal saline, or lactated Ringer's solution at a steady rate for 4 hours, after which brain tissue was collected. Brain slices were prepared and analyzed using IMS. RESULTS: CONCLUSIONS: IMS analysis in a rat craniotomy model indicated that the level of water retention in brain cells, calculated from the signal intensity of Na-adducted phosphatidylcholine around the wound area, was lowest in the Artcereb group, suggesting that artificial CSF that has similar composition and properties to human CSF can minimize edema in the brain surrounding the surgical wound.
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Authors | Shinichiro Koizumi, Takahiro Hayasaka, Naoko Goto-Inoue, Kazuhisa Doi, Mitsutoshi Setou, Hiroki Namba |
Journal | World neurosurgery
(World Neurosurg)
Vol. 77
Issue 1
Pg. 153-9
(Jan 2012)
ISSN: 1878-8769 [Electronic] United States |
PMID | 22099548
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
- Isotonic Solutions
- Pharmaceutical Solutions
- Ringer's Lactate
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Topics |
- Animals
- Brain
(pathology)
- Brain Edema
(pathology, therapy)
- Cell Membrane
(metabolism)
- Hydrogen-Ion Concentration
- Image Processing, Computer-Assisted
- Isotonic Solutions
- Lipid Metabolism
- Magnetic Resonance Imaging
- Male
- Pharmaceutical Solutions
(analysis, therapeutic use)
- Postoperative Complications
(pathology, therapy)
- Rats
- Rats, Sprague-Dawley
- Ringer's Lactate
- Therapeutic Irrigation
(methods)
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