Transmissible gastroenteritis coronavirus RNA-dependent RNA polymerase and nonstructural proteins 2, 3, and 8 are incorporated into viral particles.

Abstract

Coronavirus replication and transcription are processes mediated by a protein complex, with the RNA-dependent RNA polymerase (RdRp) as a main component. Proteomic analysis of highly purified transmissible gastroenteritis virus showed the RdRp to be a component of the viral particles. This finding was confirmed by Western blotting, immunofluorescence, and immunoelectron microscopy analyses. Interestingly, the replicase nonstructural proteins 2, 3, and 8 colocalized with the RdRp in the viral factories and were also incorporated into the virions.

• Authors: Aitor Nogales, Silvia Márquez-Jurado, Carmen Galán, Luis Enjuanes, Fernando Almazán
• Journal: Journal of virology (J Virol) Vol. 86 Issue 2 Pg. 1261-6 (Jan 2012) ISSN: 1098-5514 [Electronic] United States
• PMID: 22090122 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Viral Nonstructural Proteins
• RNA-Dependent RNA Polymerase

Topics

• Animals
• Gastroenteritis, Transmissible, of Swine (virology)
• RNA-Dependent RNA Polymerase (genetics, metabolism)
• Swine
• Transmissible gastroenteritis virus (enzymology, genetics, physiology)
• Viral Nonstructural Proteins (genetics, metabolism)
• Virion (enzymology, genetics, physiology)
• Virus Assembly
• Virus Replication