Abstract | BACKGROUND: METHODS: SPCA1 cells were pretreated with A23187 at different concentrations. The expression of GRP78 at the mRNA level was analyzed by RT-PCR; the expression of GRP78 at the protein level was determined by Western blotting and immunofluorescence assay. Cell survival was determined by MTT assay. Cell apoptosis was analyzed by flow cytometry. RESULTS: The expression of GRP78 at both the mRNA and protein levels was obviously induced by A23187 in SPCA1 cells, with an elevation of GRP78 by 2.1-fold at the mRNA level and by 3.8-fold at the protein level compared to the control. There was a dose-dependent response. Survival curve analysis demonstrated that A23187 induction caused a significant reduction of survival for the cells subjected to cisplatin treatment (P < 0.05). After treatment by cisplatin, the percentage of apoptotic cells in the A23187 pretreated group increased about three fold compared with the control group ((27.53 ± 4.32)% vs. (9.25 ± 3.64)%, P < 0.05). CONCLUSIONS:
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Authors | Li-Chuan Zhang, Jia-Rui Wang, Long Zhao, Tao Wang, Jing Wu, Su-Fang Fan, Li-Xia Chen, Shu-Juan Shao, Joseph Molnár, Qi Wang |
Journal | Chinese medical journal
(Chin Med J (Engl))
Vol. 124
Issue 20
Pg. 3341-6
(Oct 2011)
ISSN: 2542-5641 [Electronic] China |
PMID | 22088532
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Endoplasmic Reticulum Chaperone BiP
- HSPA5 protein, human
- Heat-Shock Proteins
- Calcimycin
- Cisplatin
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Calcimycin
(pharmacology)
- Cell Line, Tumor
- Cisplatin
(pharmacology)
- Endoplasmic Reticulum Chaperone BiP
- Flow Cytometry
- Heat-Shock Proteins
(genetics, metabolism)
- Humans
- Lung Neoplasms
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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