[Pulmonary lymphomatoid granulomatosis: an immunohistochemical and gene rearrangement study].

Abstract

OBJECTIVE:

To study the immunophenotype and gene rearrangement pattern of pulmonary lymphomatoid granulomatosis.

METHODS:

Nine cases of pulmonary lymphomatoid granulomatosis, included 5 cases of open lung biopsy, 3 cases of lobectomy specimen and 1 case of autopsy, were retrospectively analyzed by immunohistochemistry, in-situ hybridization for Epstein-Barr virus-encoded RNA, immunoglobulin and T-cell receptor gene rearrangement studies.

RESULTS:

The age of patients ranged from 3 to 59 years. The male-to-female ratio was 3: 6. Histologically, all cases showed lymphocytic infiltration surrounding the blood vessels and in the perivascular areas. Most of these lymphoid cells expressed T-cell marker CD3. There were also variable numbers of CD20-positive B cells. The staining for CD56 was negative. According to the WHO classification, there were 4 cases of grade I , 1 case of grade II and 4 cases of grade III lesions. Six cases had gene rearrangement studies performed and 3 of them demonstrated clonal immunoglobulin gene rearrangement (including 1 of the grade II and 2 of the grade III lesions). No T-cell receptor gene rearrangement was detected.

CONCLUSIONS:

Pulmonary lymphomatoid granulomatosis may represent a heterogeneous group of lymphoproliferative disorders. Some of the cases show B-cell immunophenotype and clonal immunoglobulin gene rearrangement, especially the grade II and grade lesions. They are likely of lymphomatous nature.

• Authors: Rui-e Feng, Hong-rui Liu, Tong-hua Liu, Jie Chen, Qing Ling, Xiao-hua Shi, Ding-rong Zhong, Yu-feng Luo, Jin-ling Cao
• Journal: Zhonghua bing li xue za zhi = Chinese journal of pathology (Zhonghua Bing Li Xue Za Zhi) Vol. 40 Issue 7 Pg. 460-4 (Jul 2011) ISSN: 0529-5807 [Print] China
• PMID: 22088372 (Publication Type: Journal Article)

Chemical References

• Antigens, CD20
• CD3 Complex

Topics

• Adult
• Antigens, CD20 (metabolism)
• CD3 Complex (metabolism)
• Child
• Child, Preschool
• Female
• Follow-Up Studies
• Gene Rearrangement, B-Lymphocyte, Heavy Chain
• Humans
• Immunohistochemistry
• Lung Neoplasms (genetics, metabolism, pathology, surgery)
• Lymphomatoid Granulomatosis (genetics, metabolism, pathology, surgery)
• Male
• Middle Aged
• Neoplasm Grading
• Pneumonectomy (methods)
• Retrospective Studies
• Young Adult