Abstract |
This study aimed to evaluate 5 enzyme immunoassays for detecting human antibodies against Taenia solium in human serum and for the diagnosis of neurocysticercosis (NCC): DRG™, RIDASCREEN™, NOVATECH™, CYPRESS™, and IVD™. A collection of 114 reference serum samples were used. All sera were tested both by ELISA and by an immunoblot method ( enzyme-linked immunoelectrotransfer blot [EITB]). When compared with EITB, the Ridascreen™ test had the best positive concordance rate (85.1-91.2%) and the NovaLisa test™ showed the optimal negative concordance rate (93.7-95.6%). All tests had a sensitivity under 72% and a specificity above 60%. The best sensitivity was obtained using Ridascreen™ test (71.4%). An optimal specificity was achieved by the NovaLisa test™. T. solium-positive sera all cross-reacted with E. granulosus positive samples. In the commercial assays evaluated here, the most appropriate ELISA test for screening may be the Ridascreen™ assay. Antibody detection seems to be not appropriate for NCC diagnosis because of its overall lack of sensitivity.
|
Authors | Jean-François Carod, Mickaël Randrianarison, Julien Razafimahefa, Rondro Mamitiana Ramahefarisoa, Mahenintsoa Rakotondrazaka, Monique Debruyne, Mélanie Dautigny, Pierrette Cazal, Marcellin Lalaoarisoa Andriantseheno, Emile Ramarokoto Charles |
Journal | Diagnostic microbiology and infectious disease
(Diagn Microbiol Infect Dis)
Vol. 72
Issue 1
Pg. 85-9
(Jan 2012)
ISSN: 1879-0070 [Electronic] United States |
PMID | 22085773
(Publication Type: Comparative Study, Evaluation Study, Journal Article)
|
Copyright | Copyright © 2012 Elsevier Inc. All rights reserved. |
Chemical References |
|
Topics |
- Animals
- Antibodies, Helminth
(blood)
- Humans
- Immunoenzyme Techniques
(methods)
- Neurocysticercosis
(diagnosis)
- Sensitivity and Specificity
- Taenia solium
(immunology, isolation & purification)
|