NADPH-diaphorase expression in the rat jejunum after intestinal ischemia/reperfusion.

Abstract

The purpose of this study was to analyze the nicotinamide adenine dinucleotide phosphate - diaphorase (NADPH-d) activity in the rat jejunum after a mesenteric ischemia/reperfusion injury. Nitric oxide, synthetised from L-arginine by the enzyme nitric oxide synthase, is a nonadrenergic noncholinergic relaxant neurotransmitter of the intestinal smooth muscle. It plays an important role in the process of plasticity after the ischemia/reperfusion injury. Experimental animals were divided in two groups: the control group and the ischemic/reperfusion group, with different period of the reperfusion. The NADPH-d histochemical method has been used as a marker for the nitric oxide synthase. NADPH-d activity has been rapidly decreased in the neurons of both enteric nervous systems in plexuses of the jejunum after 1 h mesenteric ischemia and 1 h reperfusion. Differences were predominantly detected in the myenteric plexus; they were seen in change of the neuronal shape, in the arrangement of neurons and in intensity of their staining. The NADPH-d positivity was absent in the intestinal crypts. After 1 h ischemia and 24 h reperfusion, the NADPH-d activity was gradually increased, but it was lower in comparison with the control group. On the 30th day following the ischemia/reperfusion there were no changes in NADPH-d positivity compared with the control animals. These results indicated that the jejunal ischemia/reperfusion has affected the neurons of the enteric nervous system of adult rats and resulted in the early decrease of NADPH-d positivity 1 h of the reperfusion insult. The gradual increasing of NADPH-d activity in 24 h following the reperfusion could be considered as a result of the plasticity process. On the 30(th) day after the ischemia/reperfusion all histochemical changes were returned to the control levels.

• Authors: A Bolekova, T Spakovska, D Kluchova, S Toth, J Vesela
• Journal: European journal of histochemistry : EJH (Eur J Histochem) Vol. 55 Issue 3 Pg. e23 ( 2011) ISSN: 2038-8306 [Electronic] Italy
• PMID: 22073370 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Nerve Tissue Proteins
• NADPH Dehydrogenase

Topics

• Animals
• Enteric Nervous System (enzymology, pathology)
• Gene Expression Regulation, Enzymologic
• Jejunum (innervation, pathology)
• Male
• NADPH Dehydrogenase (biosynthesis)
• Nerve Tissue Proteins (biosynthesis)
• Neurons (enzymology, pathology)
• Rats
• Rats, Wistar
• Reperfusion Injury (enzymology, pathology)
• Time Factors