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A longer tracheal occlusion period results in increased lung growth in the nitrofen rat model.

AbstractOBJECTIVE:
Prenatal tracheal occlusion (TO) promotes lung growth and is applied clinically in fetuses with severe congenital diaphragmatic hernia. Limited data are available regarding the effect of duration of TO on lung development. Our objective was to evaluate the effects of long (2 and 2.5 days) versus short (1 day) TO on lung development in rats with nitrofen-induced diaphragmatic hernia.
METHOD:
Nitrofen was administered on embryonic day (ED) 9 and fetal TO performed either on ED18.5, 19 or 20 (term = 22 days). Sham-operated and untouched littermates served as controls. On ED21, lungs were harvested and only fetuses with a left-sided diaphragmatic defect were included in further analyses.
RESULTS:
Lung-body-weight ratio incrementally increased with the duration of TO. Increased proliferation following long TO was confirmed by immunohistochemistry and qRT-PCR for the proliferation marker Ki-67. Irrespective of duration, TO induced more complex airway architecture. Medial wall thickness of pulmonary arteries was thinner after long rather than short TO.
CONCLUSION:
In the nitrofen rat model of congenital diaphragmatic hernia, a longer period of TO leads to enhanced lung growth and less muscularized pulmonary arteries.
AuthorsVeronika Beck, Marcus G Davey, Steffi Mayer, Guy Froyen, Sebastiaan Deckx, Philipp Klaritsch, Xenia I Roubliova, Scott G Petersen, Jan A Deprest
JournalPrenatal diagnosis (Prenat Diagn) Vol. 32 Issue 1 Pg. 39-44 (Jan 2012) ISSN: 1097-0223 [Electronic] England
PMID22052745 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright© 2011 John Wiley & Sons, Ltd.
Chemical References
  • Herbicides
  • Phenyl Ethers
  • nitrofen
Topics
  • Animals
  • Cell Proliferation (drug effects)
  • Disease Models, Animal
  • Female
  • Fetal Diseases (surgery)
  • Fetus (abnormalities, surgery)
  • Gestational Age
  • Herbicides (toxicity)
  • Hernia, Diaphragmatic (chemically induced, pathology, surgery)
  • Hernias, Diaphragmatic, Congenital
  • Lung (drug effects, embryology, pathology)
  • Organ Size
  • Phenyl Ethers (toxicity)
  • Pregnancy
  • Rats
  • Rats, Wistar
  • Time Factors
  • Trachea (surgery)

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