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Antigenic characterization of expressed complete and different truncated recombinant nucleocapsid proteins of hantaan virus by monoclonal antibodies.

Abstract
The nucleocapsid protein (NP) of hantavirus has been shown to be highly immunogenic in laboratory animals and humans, and it induces an early and long-lasting humoral immune response during hantavirus infection. In the present study, entire and three partially truncated open reading frames (ORF) of NP genes of Hantaan virus (HTNV) strain 76-118, a prototype of hantavirus, were amplified and then cloned in prokaryotic expression vectors. The recombinant whole NP and truncated NPs were expressed in Escherichia coli. In addition, their antigenic and biological activities were evaluated in an enzyme-linked immunosorbent assay (ELISA) for detection of a series of monoclonal antibodies (MAbs). Results showed that the expressed complete HTNV NP had a similar function as an authentic viral NP. The antigen sites on NP of hantavirus were mainly located in the N-terminus, and the region at 1-37 amino acid might be one important antigenic domain.
AuthorsXin Lü, Fanglin Zhang, Yucheng Li, Xiaoping Xue, Wen Yin, Zhikai Xu
JournalHybridoma (2005) (Hybridoma (Larchmt)) Vol. 30 Issue 5 Pg. 445-50 (Oct 2011) ISSN: 1557-8348 [Electronic] United States
PMID22008071 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Monoclonal, Murine-Derived
  • Antigens, Viral
  • Nucleocapsid Proteins
  • Peptide Fragments
  • Recombinant Proteins
Topics
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal, Murine-Derived (chemistry)
  • Antibody Specificity
  • Antigens, Viral (chemistry, immunology, metabolism)
  • Cloning, Molecular
  • Hantaan virus (immunology)
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Nucleocapsid Proteins (chemistry, immunology, metabolism)
  • Peptide Fragments (chemistry, immunology, metabolism)
  • Recombinant Proteins (chemistry, immunology, metabolism)

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