Involvement of CLEC16A in activation of astrocytes after LPS treated.

Abstract

CLEC16A, C-type lectin domain family 16, member A was recently found to be associated with inflation process in the autoimmune diseases. In this study, we elucidated the dynamic expression changes and localization of CLEC16A in lipopolysaccharide (LPS)-induced neuroinflammatory processes in adult rats. CLEC16A expression was strongly induced in active astrocytes in inflamed cerebral cortex. In vitro studies indicated that the up-regulation of CLEC16A may be involved in the subsequent astrocyte activation following LPS challenge. And Knock-down of CLEC16A in cultured primary astrocytes by siRNA showed that CLEC16A was required for the activation of astrocytes induced by LPS. Collectively, these results suggested CLEC16A may be important in host defense in astrocyte-mediated immune response. Understanding the cell signal pathway may provide a novel strategy against inflammatory and immune reaction in neuroinflammtion in CNS.

• Authors: Xinmin Wu, Jing Li, Chun Chen, Yaohua Yan, Shengyang Jiang, Xiaohong Wu, Bai Shao, Jin Xu, Lihua Kang, Yeqing Huang, Lin Zhu, Yuhong Ji, Yilu Gao
• Journal: Neurochemical research (Neurochem Res) Vol. 37 Issue 1 Pg. 5-14 (Jan 2012) ISSN: 1573-6903 [Electronic] United States
• PMID: 22002632 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CLEC16A protein, rat
• DNA Primers
• Lectins, C-Type
• Lipopolysaccharides
• Monosaccharide Transport Proteins

Topics

• Animals
• Astrocytes (drug effects, metabolism)
• Base Sequence
• Blotting, Western
• DNA Primers
• Fluorescent Antibody Technique
• Lectins, C-Type (metabolism)
• Lipopolysaccharides (toxicity)
• Male
• Monosaccharide Transport Proteins (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction