The evolution of the
mineral constituents of subcutaneous
calcinosis induced in rats by topical
calciphylaxis was studied by the method of quantitative chemical analysis, and
after treatment with excited
gases by electron spin resonance (ESR) analysis. Chemical data show that the genesis of the subcutaneous
calcinosis does not significantly alter the concentration of Ca, P, F, CO3, Mg, and Fe in the
mineral phase of the femoral bone of calciphylactic rats. In the
calcinosis an important increase of the
fluoride concentration is noticed in function of the time after challenging. There is also a high concentration of Mg2+
ions in the early stages of the experimental calcification.
Iron injected for the challenging is continuously present in the calciphylactic tissue after this treatment. This suggests that subcutaneous
calcinosis might be a means of fixing certain
heavy metal ions.
After treatment with excited
gases, the proportions of the trapped CO33- and O3- radicals are of the same order of magnitude in calciphylactic tissue after 12 days and observations in bone
mineral. These suggest that after 12 days the
mineral of the calciphylactic tissue has a crystalline state close to that of bone.