Freewheel training alters mouse hippocampal cytokines.

Abstract

The effects of 16 weeks of voluntary wheel running in healthy female mice on hippocampal expression of pro-(TNF-α, IL-1β, IL-12) and anti-(IL-10, IL-1ra) inflammatory and pleiotropic (IL-6) cytokines and apoptotic status of specific cell subsets (CD45+, CD11b+) were studied. Mice were assigned to wheel running (WR; n=20) or a control condition (No WR; n=22) and sacrificed after the 16 weeks. Data collected included measures of training status (running volume, body weight, run-to-exhaustion time, and skeletal muscle cytochrome c oxidase activity), flow cytometric analysis of cell phenotypes and apoptosis (CD45+, CD11b+, Annexin+, Annexin+/PI+, PI+), and cytokine concentrations in cell lysates. WR mice had measurable training effects and significantly lower TNF-α (p<0.05) and higher IL-6 (p<0.05), IL-1ra (p<0.05) and IL-12 (p<0.05) expression in the hippocampus compared to controls. IL-1β, IL-10, and the percent of apoptotic and dead cells did not change due to training. Taken together, and in relation to the complex interactions between cytokines, the results suggest a possible mechanism whereby exercise training may buffer from dementia and cognitive decline through changes in the central cytokine milieu in the hippocampus.

• Authors: N Pervaiz, L Hoffman-Goetz
• Journal: International journal of sports medicine (Int J Sports Med) Vol. 32 Issue 11 Pg. 889-95 (Nov 2011) ISSN: 1439-3964 [Electronic] Germany
• PMID: 21989671 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• Cytokines
• Electron Transport Complex IV

Topics

• Animals
• Apoptosis (physiology)
• Body Weight
• Cytokines (metabolism)
• Electron Transport Complex IV (metabolism)
• Female
• Flow Cytometry
• Hippocampus (metabolism)
• Mice
• Mice, Inbred C57BL
• Muscle, Skeletal (metabolism)
• Physical Conditioning, Animal (physiology)
• Running (physiology)