Abstract |
This study aimed to shed light on the anti-inflammatory and hepatoprotective effect of the major alkamides dodeca-2E,4E,8Z,10Z(E)-tetraenoic acid isobutylamides (Alk-8/9), isolated from Echinacea purpurea roots, against acute fulminant hepatitis induced by lipopolysaccharide/D- galactosamine (LPS/D-GalN) in mice. The results show that Alk-8/9 dose-dependently induced heme oxygenase (HO)-1 protein expression in LPS-stimulated murine macrophages that was likely regulated by the JNK-mediated pathway through increasing SAPK/JNK phosphorylation, c-jun protein expression, and phosphorylation, and transcription factor AP-1 binding consensus DNA activity. The HO-1 inhibitor or CO scavenger significantly reversed the inhibitory effect of Alk-8/9 on TNF-α expression, whereas N-acetyl-L-cysteine was observed to reduce Alk-8/9-induced HO-1 expression in LPS-treated macrophages. Furthermore, Alk-8/9 markedly induced c-jun and HO-1 protein expression and suppressed serum aminotransferase activities, TNF-α expression, and hepatocyte damage in liver tissues of LPS/d-GalN-treated mice. This paper suggests a new application of Echinacea, a top-selling herbal supplement, as a hepatoprotective agent.
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Authors | Chia-Chung Hou, Chi-Chang Huang, Lie-Fen Shyur |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 59
Issue 22
Pg. 11966-74
(Nov 23 2011)
ISSN: 1520-5118 [Electronic] United States |
PMID | 21985290
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Lipopolysaccharides
- Plant Extracts
- Polyunsaturated Alkamides
- Galactosamine
- Heme Oxygenase-1
- MAP Kinase Kinase 4
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Topics |
- Animals
- Echinacea
(chemistry)
- Galactosamine
(adverse effects)
- Gene Expression
(drug effects)
- Heme Oxygenase-1
(genetics, metabolism)
- Hepatitis
(drug therapy, enzymology, prevention & control)
- Humans
- Lipopolysaccharides
(adverse effects)
- Liver
(drug effects, enzymology, injuries)
- MAP Kinase Kinase 4
(genetics, metabolism)
- Male
- Mice
- Mice, Inbred ICR
- Plant Extracts
(administration & dosage)
- Polyunsaturated Alkamides
(administration & dosage)
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