TFPI-2 methylation predicts poor prognosis in non-small cell lung cancer.

Abstract

BACKGROUND:

Methylation of human tissue factor pathway inhibitor-2 (TFPI-2) gene has been detected in several types of cancer, including non-small cell lung cancer (NSCLC). However, an association between the methylation status of TFPI-2 gene and prognosis has not yet been investigated.

METHODS:

Methylation of TFPI-2 gene was examined in a consecutive series of 133 non-metastatic NSCLC patients using methylation-specific PCR (MSP). Univariate and multivariate analyses were conducted to investigate the association between clinical variables and overall survival time.

RESULTS:

Methylation of TFPI-2 gene was detected in 36 of 133 patients (27.1%). Of these 36 patients, seventeen individuals (47.2%) carried stage III tumors. The 5-year disease free survival rate among patients carrying methylated TFPI-2 tumors was significantly lower as compared to those with unmethylated TFPI-2 tumors (35.5% versus 6.1%, P<0.0001). Moreover, methylation of TFPI-2 gene was found to be an independent prognostic factor for poor overall survival based on multivariate analysis models (P=0.013), as was age >62 years old (P<0.0001) and TNM stage of disease (P<0.0001).

CONCLUSIONS:

The results of the present study suggest that methylation of TFPI-2 gene is an independent factor for an unfavorable prognosis in patients with NSCLC.

• Authors: Duoguang Wu, Lihua Xiong, Shuoming Wu, Ming Jiang, Guiyong Lian, Minghui Wang
• Journal: Lung cancer (Amsterdam, Netherlands) (Lung Cancer) Vol. 76 Issue 1 Pg. 106-11 (Apr 2012) ISSN: 1872-8332 [Electronic] Ireland
• PMID: 21983100 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• DNA, Neoplasm
• Glycoproteins
• RNA, Neoplasm
• tissue-factor-pathway inhibitor 2

Topics

• Adenocarcinoma (genetics, mortality, pathology)
• Carcinoma, Large Cell (genetics, mortality, pathology)
• Carcinoma, Non-Small-Cell Lung (genetics, mortality, pathology)
• Carcinoma, Squamous Cell (genetics, mortality, pathology)
• DNA Methylation
• DNA, Neoplasm (genetics)
• Female
• Follow-Up Studies
• Glycoproteins (genetics)
• Humans
• Lung Neoplasms (genetics, mortality, pathology)
• Male
• Middle Aged
• Neoplasm Staging
• Prognosis
• RNA, Neoplasm (genetics)
• Real-Time Polymerase Chain Reaction
• Survival Rate