[Immunogenicity of sabin inactivated poliovirus vaccine induced by diphtheria-tetanus-acellular pertussis and Sabin inactivated poliovirus combined vaccine].

Abstract

OBJECTIVE:

In order to search the preparation process and optimazing dosage ratio of adsorbed diphtheria-tetanus-acellular pertussis and sabin inactivated poliovirus combined vaccine (DTaP-sIPV), the neutralizing antibody titers of IPV induced by different concentration of DTaP-sIPV were investigated on rats.

METHODS:

Two batches of DTaP-sLPV were produced using different concentration of sIPV and the quality control was carried. Together with sabin-IPV and DTaP-wIPV ( boostrix-polio, GSK, Belgium) as control group, the DTaP-sIPV were administrated on three-dose schedule at 0, 1, 2 month on rats. Serum sample were collected 30 days after each dose and neutralizing antibody titers against three types poliovirus were determined using micro-neutralization test.

RESULTS:

Two batches of prepared DTaP-sIPV and control sLPV were according to the requirement of Chinese Pharmacopoeia (Volume III, 2005 edition) and showed good stability. The seropositivity rates were 100% for sabin inactivated poliovirus antigen in all groups. The GMTs (Geometric mean titers) of neutralizing antibodies against three types poliovirus increased.

CONCLUSION:

The prepared DTaP-sIPV was safe, stable and effective and could induced high level neutralizing antibody against poliovirus on rats.

• Authors: Yan Ma, Min Qin, Hui-Qiong Hu, Guang Ji, Ling Feng, Na Gao, Jie Gu, Bing-Feng Xie, Ji-Hong He, Ming-Bo Sun
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 25 Issue 3 Pg. 197-200 (Jun 2011) ISSN: 1003-9279 [Print] China
• PMID: 21977591 (Publication Type: English Abstract, Journal Article)

Chemical References

• Antibodies, Viral
• Diphtheria-Tetanus-acellular Pertussis Vaccines
• Poliovirus Vaccine, Inactivated
• Vaccines, Combined

Topics

• Animals
• Antibodies, Viral (immunology)
• Diphtheria-Tetanus-acellular Pertussis Vaccines (immunology)
• Female
• Male
• Poliovirus Vaccine, Inactivated (immunology)
• Rats
• Rats, Wistar
• Vaccines, Combined (immunology)