Combined fish oil and astaxanthin supplementation modulates rat lymphocyte function.

Abstract

PURPOSE:

Higher intakes of n-3 polyunsaturated fatty acids that are abundant in marine fishes have been long described as a "good nutritional intervention" with increasing clinical benefits to cardiovascular health, inflammation, mental, and neurodegenerative diseases. The present study was designed to investigate the effect of daily fish oil (FO-10 mg EPA/kg body weight (BW) and 7 mg DHA/kg BW) intake by oral gavage associated with the antioxidant astaxanthin (ASTA-1 mg/kg BW) on the redox metabolism and the functional properties of lymphocytes from rat lymph nodes.

METHODS:

This study was conducted by measurements of lymphocyte proliferation capacity, ROS production [superoxide (O₂(•-)) and hydrogen peroxide (H₂O₂)], nitric oxide (NO(•)) generation, intracellular calcium release, oxidative damage to lipids and proteins, activities of major antioxidant enzymes, GSH/GSSG content, and cytokines release.

RESULTS:

After 45 days of FO + ASTA supplementation, the proliferation capacity of activated T- and B-lymphocytes was significantly diminished followed by lower levels of O₂(•-), H₂O₂ and NO(•) production, and increased activities of total/SOD, GR and GPx, and calcium release in cytosol. ASTA was able to prevent oxidative modification in cell structures through the suppression of the oxidative stress condition imposed by FO. L: -selectin was increased by FO, and IL-1β was decreased only by ASTA supplementation.

CONCLUSION:

We can propose that association of ASTA with FO could be a good strategy to prevent oxidative stress induced by polyunsaturated fatty acids and also to potentiate immuno-modulatory effects of FO.

• Authors: Rosemari Otton, Douglas Popp Marin, Anaysa Paola Bolin, Rita de Cássia Santos Macedo, Thais Regina Campoio, Claudio Fineto Jr, Beatriz Alves Guerra, José Roberto Leite, Marcelo Paes Barros, Rita Mattei
• Journal: European journal of nutrition (Eur J Nutr) Vol. 51 Issue 6 Pg. 707-18 (Sep 2012) ISSN: 1436-6215 [Electronic] Germany
• PMID: 21972007 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Cytokines
• Fish Oils
• Mitogens
• Reactive Oxygen Species
• Xanthophylls
• Nitric Oxide
• astaxanthine
• Oxidoreductases
• Glutathione

Topics

• Animals
• Antioxidants (administration & dosage)
• Calcium Signaling (drug effects)
• Cell Proliferation (drug effects)
• Cells, Cultured
• Cytokines (metabolism)
• Dietary Supplements
• Fish Oils (administration & dosage, chemistry)
• Glutathione (metabolism)
• Immunomodulation
• Lipid Peroxidation (drug effects)
• Lymphocytes (drug effects, immunology, metabolism)
• Male
• Mitogens (pharmacology)
• Nitric Oxide (metabolism)
• Oxidation-Reduction
• Oxidative Stress (drug effects)
• Oxidoreductases (metabolism)
• Rats
• Rats, Wistar
• Reactive Oxygen Species (metabolism)
• Xanthophylls (administration & dosage)